RT Journal Article
T1 Oral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression
A1 Crespo Vieira, Andrés
A1 Rubiolo Gaytán, Juan Andrés
A1 López Alonso, Henar
A1 Cifuentes Martínez, José Manuel
A1 Alfonso Rancaño, María Amparo
A1 Bermúdez Pose, Roberto
A1 Otero Fuertes, María Paz
A1 Rodríguez Vieytes, Mercedes
A1 Vega Lisi, Félix Víctor
A1 Botana López, Luis Miguel
K1 Okadaic acid
K1 Histopathology
K1 Diarrhea
K1 Immunohistochemistry
K1 Liver
K1 Superoxide dismutase 1
K1 Catalase
K1 Quantitative PCR
K1 Mice
AB In vivo, after administration by gavage to mice and rats, okadaic acid has been reported to produce lesions in liver, small intestine and forestomach. Because several reports differ in the damage detected in different organs, and on okadaic acid distribution after consumption, we determined the toxicity of this compound after oral administration to mice. After 24 hours, histopathological examination showed necrotic foci and lipid vacuoles in the livers of intoxicated animals. By immunohistochemical analysis, we detected this toxin in the liver and kidneys of intoxicated animals. Okadaic acid induces oxidative stress and can be activated in vitro into reactive compounds by the post-mitochondrial S9 fraction, so we studied the okadaic effect on the gene expression of antioxidant and phase II detoxifying enzymes in liver. We observed a downregulation in the expression of these enzymes and a reduction of protein expression of catalase and superoxide dismutase 1 in intoxicated animals
PB MDPI
YR 2013
FD 2013
LK http://hdl.handle.net/10347/23597
UL http://hdl.handle.net/10347/23597
LA eng
NO Vieira, A.C.; Rubiolo, J.A.; López-Alonso, H.; Cifuentes, J.M.; Alfonso, A.; Bermúdez, R.; Otero, P.; Vieytes, M.R.; Vega, F.V.; Botana, L.M. Oral Toxicity of Okadaic Acid in Mice: Study of Lethality, Organ Damage, Distribution and Effects on Detoxifying Gene Expression. Toxins 2013, 5, 2093-2108
NO The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR.From the European Union’s Seventh Framework Programme managed by REA–Research Executive Agency (FP7/2007-2013) under grant agreement Nos. 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2009-1/117 Pharmatlantic
DS Minerva
RD 24 abr 2026