RT Book,_Section
T1 Toxicological studies with cells
A1 Vale González, María del Carmen
A1 Boente Juncal, Andrea
A1 González Méndez, Aida
A2 Botana López, Luis Miguel
K1 Cytotoxicity
K1 In vitro
K1 Neuronal cultures
K1 Immunocytochemistry
K1 MTT
K1 Western blot
AB Cell culture yields a model system that provides direct access and evaluation of the effects of chemicals on tissues and constitutes a valuable tool to analyze cell toxicity mechanisms. In vitro model systems, in general, have been used to study the mechanism of action of chemicals and to analyze the cellular basis for compound-induced toxicity as well as to develop rapid and high-throughput screening systems for the evaluation of the toxicity of chemicals, which may complement in vivo toxicity testing or may replace some in vivo models if they are scientifically validated and accepted by regulatory agencies [1, 3]. The term in vitro (“in the glass”) refers to the technique of performing a given experiment in a test tube or in a controlled environment outside a living organism. In vitro methods are based on the use of cells or tissues which are cultured under controlled conditions in flasks and plates. Cells/tissues are exposed to chemicals and their toxic or beneficial effect is measured. Increasingly, human cells are used since they better predict possible effects on humans. Commonly used in vitro models for assessing chemical toxicity include perfused organ preparations, isolated tissue preparations, single cell suspensions, and cell culture systems, such as primary cell cultures and mammalian cell lines. Of these in vitro models, cell culture systems have been widely used because they are reliable, reproducible, and relatively inexpensive experimental systems to assess chemical toxicity at the cellular level.
PB De Gruyter
SN 9783110442045
YR 2018
FD 2018-05-17
LK https://hdl.handle.net/10347/46859
UL https://hdl.handle.net/10347/46859
LA eng
NO Vale González, Carmen , Juncal, Andrea Boente and Méndez, Aida González. "5. Toxicological studies with cells". Environmental Toxicology, edited by Luis M. Botana, Berlin, Boston: De Gruyter, 2018, pp. 115-145. https://doi.org/10.1515/9783110442045-005
NO This work has received funding from the following FEDER cofunded-grants: from CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad, AGL2014-58210-R, AGL2016-78728-R (AEI/FEDER, UE), ISCIII/PI16/01830 and RTC-2016-5507-2; from CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD and ITC-20161072; and from European Union POCTEP 0161-Nanoeaters -1-E-1, and Interreg Atlantic Area Transnational Cooperation Programme 2014-2020: EAPA_317/2016, ALERTOX-NET.
DS Minerva
RD 9 may 2026