RT Journal Article
T1 2-DE-based proteomic analysis of common bean (Phaseolus vulgaris L.) seeds
A1 Fuente, M. de la
A1 Borrajo, A.
A1 Bermúdez, J.
A1 Lores Aguín, Marta
A1 Alonso, J.
A1 López-Pedrouso, María
A1 Santalla, M.
A1 Ron Pedreira, Antonio Miguel de
A1 Zapata Babío, José Carlos
A1 Álvarez, G.
K1 Phaseolus vulgaris
K1 Seed proteome
K1 Two-dimensional electrophoresis
K1 Mass spectrometry
K1 Protein extraction methods
AB Common bean (Phaseolus vulgaris L.) is the most important grain legume for direct human consumption. Proteomic studies in legumes have increased significantly in the last years but few studies have been performed to date in P. vulgaris. We report here a proteomic analysis of bean seeds by two-dimensional electrophoresis (2-DE). Three different protein extraction methods (TCA–acetone, phenol and the commercial clean-up kit) were used taking into account that the extractome can have a determinant impact on the level of quality of downstream protein separation and identification. To demonstrate the quality of the 2-DE analysis, a selection of 50 gel spots was used in protein identification by mass spectrometry (MALDI-TOF MS and MALDI-TOF/TOF). The results showed that a considerable proportion of spots (70%) were identified in spite of incomplete genome/protein databases for bean and other legume species. Most identified proteins corresponded to storage protein, carbohydrate metabolism, defense and stress response, including proteins highly abundant in the seed of P. vulgaris such as the phaseolin, the phytohemagglutinin and the lectin-related α-amylase inhibitor.
PB Elsevier
SN 1874-3919
YR 2011
FD 2011-02-01
LK https://hdl.handle.net/10347/39084
UL https://hdl.handle.net/10347/39084
LA eng
NO De La Fuente, M., Borrajo, A., Bermúdez, J., Lores, M., Alonso, J., López, M., ... & Alvarez, G. (2011). 2-DE-based proteomic analysis of common bean (Phaseolus vulgaris L.) seeds. Journal of proteomics, 74(2), 262-267.
NO The research was supported by AGL2008-02091/AGR project from the Spanish Government and EU-FEDER Funds.
DS Minerva
RD 27 abr 2026