RT Journal Article
T1 Development and characterisation of bilayered periosteum-inspired composite membranes based on sodium alginate-hydroxyapatite nanoparticles
A1 D'Elía, Noelia Laura
A1 Rial Silva, Ramón
A1 Sartuqui, Javier
A1 Ercoli, Daniel
A1 Ruso Beiras, Juan Manuel
A1 Messina, Paula V.
A1 Mestres, Gemma
K1 Alginate
K1 Bilayer
K1 Cell culture
K1 Fibroblasts
K1 Guided bone regeneration
K1 Membrane
K1 Nanohydroxyapatite
K1 Osteoblasts
K1 Periosteum
K1 Rheology
AB Background and aim: Membranes for guided bone regeneration should have a mechanical structure and a chemical composition suitable for mimicking biological structures. In this work, we pursue the development of periosteum-inspired bilayered membranes obtained by crosslinking alginate with different amounts of nanohydroxyapatite.Experiments: Alginate-nanohydroxyapatite interaction was studied by rheology and infrared spectroscopy measurements. The membranes were characterized regarding their tensile strength, degradation and surface morphology. Finally, cell cultures were performed on each side of the membranes.Findings: The ionic bonding between alginate polysaccharide networks and nanohydroxyapatite was proven, and had a clear effect in the strength and microstructure of the hydrogels. Distinct surface characteristics were achieved on each side of the membranes, resulting in a highly porous fibrous side and a mineral-rich side with higher roughness and lower porosity. Moreover, the effect of amount of nanohydroxyapatite was reflected in a decrease of the membranes’ plasticity and an increment of degradation rate. Finally, it was proved that osteoblast-like cells proliferated and differentiated on the mineral-rich side, specially when a higher amount of nanohydroxyapatite was used, whereas fibroblasts-like cells were able to proliferate on the fibrous side. These periosteum-inspired membranes are promising biomaterials for guided tissue regeneration applications
PB Elsevier
SN 0021-9797
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24308
UL http://hdl.handle.net/10347/24308
LA eng
NO Journal of Colloid and Interface Science, Volume 572, 15 July 2020, Pages 408-420
NO The authors acknowledge Universidad Nacional del Sur (PGI24/Q092), Concejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET, PIP- 11220130100100CO) Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina (PICT- 201-0126), Fundación Ramón Areces and Xunta de Galicia (ED41E2018/08). NLD and JS have postdoctoral fellowships of CONICET and PVM is independent of CONICET. GM acknowledges Swedish Foundation for International Cooperation in Research and Higher Education (STINT; #IB2017-7362), Swedish Council Formas (#2016-00781), Swedish Council Vetenskapsrådet (#2017-05051) and Göran Gustafsson Foundation (#1841) for financial support
DS Minerva
RD 24 abr 2026