RT Journal Article
T1 Avoiding acid crash: From apple pomace hydrolysate to butanol through acetone-butanol-ethanol fermentation in a zero-waste approach
A1 Bravo Venegas, Javier
A1 Prado Acebo, Inés
A1 Gullón Estévez, Beatriz
A1 Lu Chau, Thelmo Alejandro
A1 Eibes González, Gemma María
K1 ABE fermentation
K1 Clostridium beijerinckii CECT 508
K1 pH
K1 Temperature
K1 Calcium carbonate
K1 Biorefinery
AB Apple pomace (AP) is a lignocellulosic residue from the juice and cider industries that can be valorized in a multi-product biorefinery to generate multiple value-added compounds, including biofuels such as butanol. Butanol is produced biologically by acetone-butanol-ethanol (ABE) fermentation using bacteria of the genus Clostridium from sugar-based feedstocks. In this study, AP hydrolysate was used as a substrate for producing butanol by ABE fermentation. Various environmental factors influence the amount of butanol produced, but only under certain conditions the so-called 'acid crash', an undesirable phenomenon characterized by a total arrest of cell growth and solvent production, can be avoided. Operational parameters that may influence the prevention of acid crash occurrence, such as pH, CaCO3 concentration and culture temperature, were optimized in C. beijerinckii CECT 508 cultures applying a Box-Behnken experimental design. The mathematical model of the fermentation found the optimal conditions of pH 7, 6.8 g/L of CaCO3 and 30 °C, and this was validated in an independent experiment carried out at the optimal conditions, reaching 10.75 g/L of butanol. Also, the comparison of butanol production between the supernatant of the AP hydrolysate (10.57 g/L) and the full hydrolysate with solids (11.69 g/L) indicated that it is possible to eliminate the centrifugation step after hydrolysis, which may allow to reduce process costs and the full utilization of apple pomace, aiming a zero-waste approach
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30643
UL http://hdl.handle.net/10347/30643
LA eng
NO Waste Management 164 (2023) 47–56
NO This research was funded by Xunta de Galicia (ED431F 2020/06), by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future” (grants RYC2018-024846-I to G.E. and RYC2018-026177-I to B.G.) and co-financed by tax funds on the basis of the budget approved by the Saxon State Parliament (SAB N° 100393946). J.B-V, I.P-A, T.L-C and G.E. belong to the Galician Competitive Research Group (GRC) ED431C-2021/37
DS Minerva
RD 24 abr 2026