RT Journal Article
T1 Virulence patterns in a murine sepsis model of ST131 escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16: H5 are associated to specific virotypes
A1 Mora Gutiérrez, Azucena
A1 Dahbi, Ghizlane
A1 López Capón, Cecilia
A1 Mamani Huarani, Rosalía Seferina
A1 Marzoa Fandiño, Juan
A1 Dion, Sara
A1 Picard, Bertrand
A1 Blanco Álvarez, Miguel
A1 Alonso, María Pilar
A1 Denamur, Erick
A1 Blanco Álvarez, Jorge
K1 Escherichia coli
K1 Murine Sepsis
AB Escherichia coli sequence type (ST)131 is an emerging disseminated public health threat implicated in multidrug-resistant extraintestinal infections worldwide. Although the majority of ST131 isolates belong to O25b:H4 serotype, new variants with different serotypes, STs using the discriminative multilocus sequence typing scheme of Pasteur Institute, and virulence-gene profiles (virotypes) have been reported with unknown implications on the pattern of spread, persistence and virulence. Theaim of the present study was to compare virulence in a mouse subcutaneous sepsis model of representative ST131 clinical isolates belonging to 2 serotypes (O25b:H4, O16:H5) and nine virotypes and subtypes (A, B, C, D1, D2, D3, D4, D5 and E). Fourteen out of the 23 ST131 isolates tested (61%) killed 90 to 100% of mice challenged, and 18 of 23 (78%) at least 50%. Interestingly, different virulence patterns in association with virotypes were observed, from highly rapid lethality (death in less than 24 h) to low final lethality (death at 7 days) but with presence of an acute inflammation. This is the first study to assess virulence of ST131 isolates belonging to serotype O16:H5, which exhibited virotype C. In spite of their low virulencegene score, O16:H5 isolates did not show significant differences in final lethality compared with highly virulent O25b:H4 isolates of virotypes A, B and C, but killed mice less rapidly. Significant differences were found, however, between virotypesA, B, C (final lethality $80% of mice challenged) and virotypes D, E. Particularly unexpected was the low lethality of the newly assigned virotype E taking into account that it exhibited high virulence-gene score, and the same clonotype H30 as highly virulent O25b:H4 isolates of virotypes A, B and C. In vivo virulence diversity reported in this study would reflect the genetic variability within ST131 clonal group evidenced by molecular typing
PB Plos
YR 2014
FD 2014
LK http://hdl.handle.net/10347/22009
UL http://hdl.handle.net/10347/22009
LA eng
NO Mora Gutiérrez, A., Dahbi, G., López, C., Mamani, R., Marzoa J., Dion, S. et al. (2014). Virulence patterns in a murine sepsis model of ST131 escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16:H5 are associated to specific virotypes. PLoS ONE vol.9(1): e87025
NO This work was partially supported by the Red Española de Investigación en Patología Infecciosa (REIPI RD06/0008/1018-1016) and grants PI09/01273 (Instituto de Salud Carlos III, Fondo de Investigación Sanitaria, Ministerio de Economía y Competitividad, Gobierno de España), FEDER-INNTERCONECTA-COLIVAC (CDTI, Ministerio de Economía y Competitividad, Gobierno de España; Consellería de Economía e Industria, Xunta de Galicia; The European Regional Development Fund, ERDF) and CN2012/303 (Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and the ERDF)
DS Minerva
RD 30 abr 2026