RT Journal Article
T1 Synthesis of oligosaccharides derived from lactulose (OsLu) using soluble and immobilized Aspergillus oryzae β-Galactosidase
A1 Cardelle Cobas, Alejandra
A1 Olano, Agustín
A1 Irazoqui, Gabriela
A1 Giacomini, Cecilia
A1 Batista Viera, Francisco
A1 Corzo, Nieves
A1 Corzo Martínez, Marta
K1 Oligosaccharides
K1 Lactulose
K1 Aspergillus oryzae
K1 Immobilization
K1 Glutaraldehyde–agarose
AB β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction conditions affecting the specific selectivity of A. oryzae β-galactosidase for synthesis of OsLu has been carried out to enhance OsLu with DP ≥ 3 production. Assays with β-galactosidase immobilized onto a glutaraldehyde–agarose support were also carried out with the aim of making the process cost-effective and industrially viable. Optimal conditions with both soluble and immobilized enzyme for the synthesis of OsLu with DP ≥ 3 were 50 °C, pH 6.5, 450 g/L of lactulose, and 8 U/mL of enzyme, reaching yields of ca. 50% (w/v) of total OsLu and ca. 20% (w/v) of OsLu with DP 3, being 6′-galactosyl-lactulose the major one, after a short reaction time. Selective formation of disaccharides, however, was favored at 60 °C, pH 4.5, 450 g/L of lactulose and 8 U/mL of enzyme. Immobilization increased the enzymatic stability to temperature changes and allowed to reuse the enzyme. We can conclude that the use, under determined optimal conditions, of the A. oryzae β-galactosidase immobilized on a support of glutaraldehyde–agarose constitutes an efficient and cost-effective alternative to the use of soluble β-galactosidases for the synthesis of prebiotic OsLu mixtures.
PB Frontiers Media
YR 2016
FD 2016
LK http://hdl.handle.net/10347/22391
UL http://hdl.handle.net/10347/22391
LA eng
NO Cardelle-Cobas A, Olano A, Irazoqui G, Giacomini C, Batista-Viera F, Corzo N and Corzo-Martínez M (2016) Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase. Front. Bioeng. Biotechnol. 4:21. doi: 10.3389/fbioe.2016.00021
NO This work has been supported by projects AGL2014-53445-R from Ministerio de Economía y Competitividad; ALIBIRD-CM S-2013/ABI-2728 from Comunidad Autónoma de Madrid and COOPB20099 from CSIC
DS Minerva
RD 24 abr 2026