RT Journal Article T1 Factors Affecting Fatty Acid Composition of Holstein Cow’s Milk A1 Rodríguez Bermúdez, Ruth A1 Fouz Dopacio, Ramiro A1 Camino, Fernando A1 Souza, Taile Katiele A1 Miranda Castañón, Marta Inés A1 Rico Gómez, Margarita A1 Diéguez Casalta, Javier K1 Fatty acids concentration K1 Dairy cattle K1 Mixed effects ordinal regression K1 Dairy farming K1 Raw milk quality K1 Methane emissions AB Milk fatty acid composition has gained the interest of both manufacturers and consumers during recent years. The present paper aimed to perform an analysis of C14:0, C16:0, C18:0, C18:1, saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA) and short chain fatty acid (SCFA) concentration in cow’s milk in relation to the type of ration, parity, lactation phase and season. Cows’ milk from animals being fed total mixed rations, including corn silage, had higher C14:0, C16:0 and SFA concentrations than those being fed pasture-based rations but lower concentrations of C18:0 and PUFA. Comparing to 1st parity cows, 2nd and 3rd parity animals had higher SFA and SCFA concentrations in milk. With respect to spring, C14:0, C16:0 and SFA concentrations increased in summer, autumn and winter while MUFA, PUFA and SCFA concentrations decreased. Considering the lactation phase, C14:0, C16:0 and SFA concentrations decreased in fresh cows with ketosis comparing to healthy fresh cows and increased in peak, mid and late lactation. C18:0, C18:1 and MUFA follow the opposite trend. The milk fatty acid profile varies significantly through the studied effects. The fact that the fatty acid profile is associated with animal health, organoleptic properties of milk or even methane production highlights the importance of studying factors thataffect its variation. PB MDPI YR 2023 FD 2023 LK http://hdl.handle.net/10347/32722 UL http://hdl.handle.net/10347/32722 LA eng NO Rodríguez-Bermúdez, R., Fouz, R., Rico, M., Camino, F., Souza, T. K., Miranda, M. & Diéguez, F. J. (2023). Factors affecting fatty acid composition of holstein cow’s milk. Animals (Basel), 13(4), 574-. https://doi.org/10.3390/ani13040574 NO This research was funded by European Agricultural Fund for Rural Development (EAFRD),grant number 2016/59B. DS Minerva RD 3 may 2026