RT Journal Article
T1 Exploration of JAK/STAT pathway activation in ulcerative colitis reveals sex-dependent activation of JAK2/STAT3 in the inflammatory response
A1 Calviño-Suárez, Cristina
A1 Durán Rubí, Mariña
A1 Brea Floriani, José Manuel
A1 Moreira Álvarez, David
A1 Ardao Palacios, Inés
A1 Brocos Mosquera, Iria
A1 Ferreiro Iglesias, Rocío
A1 Porto Silva, Sol
A1 Nieto-García, Laura
A1 Varela Liste, María José
A1 Loza García, María Isabel
A1 Martínez Rodríguez, Antón Leandro
A1 Barreiro de Acosta, Manuel
K1 Ulcerative colitis
K1 JAK/STAT pathway
K1 Phosphorylation
K1 Sex-specific differences
K1 Inflammatory signaling
K1 Personalized therapy
AB Introduction. Ulcerative colitis (UC) is characterized by aberrant immune responses involving multiple inflammatory pathways, including JAK/STAT signaling. However, the specific roles and interactions of individual components within this pathway remain unclear.Methods. We conducted a prospective, observational, single-center study enrolling 61 adult UC patients undergoing routine colonoscopy with endoscopic activity (Mayo Endoscopic Score > 0). Paired biopsies from inflamed and non-inflamed colonic mucosa were collected. Phosphorylation levels of JAK1, JAK2, JAK3, TYK2, STAT1, STAT3, and STAT4 were quantified by Western blot.Results. Inflamed tissue showed significantly increased phosphorylation of JAK2, JAK3, TYK2, STAT1, STAT3, and STAT4 compared to non-inflamed mucosa (p < 0.05), while JAK1 levels did not differ significantly. Correlation analysis revealed coordinated activation among JAK2, JAK3, TYK2, and STAT3, suggesting interdependent roles. Notably, male patients exhibited significantly higher activation of JAK2 and STAT3 than female patients (p < 0.05).Discussion. These findings highlight a heterogeneous but important involvement of the JAK/STAT pathway in UC pathophysiology. The observed sex-specific differences and coordinated activation patterns suggest the value of personalized therapeutic approaches targeting specific components of this pathway.
PB Frontiers
YR 2025
FD 2025-07-21
LK https://hdl.handle.net/10347/42564
UL https://hdl.handle.net/10347/42564
LA eng
NO Calviño-Suarez C, Duran-Rubi M, Brea J, Moreira D, Ardao I, Brocos-Mosquera I, Ferreiro-Iglesias R, Porto-Silva S, Nieto-Garcia L, Varela MJ, Loza MI, Martinez AL and Barreiro-de Acosta M (2025) Exploration of JAK/STAT pathway activation in ulcerative colitis reveals sex-dependent activation of JAK2/STAT3 in the inflammatory response. Front. Immunol. 16:1609740.
NO The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by a grant from the Fund for Health Research of Carlos III Health Institute (PI21/01220) and by the grant CPP2021-009055 funded by MICIU/AEI /10.13039/501100011033 and by European Union NextGenerationEU/ PRTR. We gratefully acknowledge grant support from Xunta de Galicia (ED431C 2022/20) and European Regional Development Fund (ERDF). MD-R was a beneficiary of an i-PFIS contract under the Acción Estratégica en Salud program of the Instituto de Salud Carlos III.
DS Minerva
RD 29 abr 2026