RT Journal Article
T1 Accuracy of periodontitis diagnosis obtained using multiple molecular biomarkers in oral fluids: A systematic review and meta‐analysis
A1 Blanco Pintos, Triana
A1 Regueira Iglesias, Alba
A1 Seijo Porto, Iria
A1 Balsa Castro, Carlos
A1 Castelo Baz, Pablo
A1 Nibali, Luigi
A1 Tomás Carmona, Inmaculada
K1 Diagnostic accuracy
K1 Meta-analysis
K1 Molecular biomarkers
K1 Oral fluids
K1 Periodontitis
AB AimTo determine the accuracy of biomarker combinations in gingival crevicular fluid (GCF) and saliva through meta-analysis to diagnose periodontitis in systemically healthy subjects.MethodsStudies on combining two or more biomarkers providing a binary classification table, sensitivity/specificity values or group sizes in subjects diagnosed with periodontitis were included. The search was performed in August 2022 through PUBMED, EMBASE, Cochrane, LILACS, SCOPUS and Web of Science. The methodological quality of the articles selected was evaluated using the QUADAS-2 checklist. Hierarchical summary receiver operating characteristic modelling was employed to perform the meta-analyses (CRD42020175021).ResultsTwenty-one combinations in GCF and 47 in saliva were evaluated. Meta-analyses were possible for six salivary combinations (median sensitivity/specificity values): IL-6 with MMP-8 (86.2%/80.5%); IL-1β with IL-6 (83.0%/83.7%); IL-1β with MMP-8 (82.7%/80.8%); MIP-1α with MMP-8 (71.0%/75.6%); IL-1β, IL-6 and MMP-8 (81.8%/84.3%); and IL-1β, IL-6, MIP-1α and MMP-8 (76.6%/79.7%).ConclusionsTwo-biomarker combinations in oral fluids show high diagnostic accuracy for periodontitis, which is not substantially improved by incorporating more biomarkers. In saliva, the dual combinations of IL-1β, IL-6 and MMP-8 have an excellent ability to detect periodontitis and a good capacity to detect non-periodontitis. Because of the limited number of biomarker combinations evaluated, further research is required to corroborate these observations
PB Wiley
SN 0303-6979
YR 2023
FD 2023-08-22
LK http://hdl.handle.net/10347/31189
UL http://hdl.handle.net/10347/31189
LA eng
NO Journal of Clinical Periodontology, 2023 50(11), 1420–1443
NO This study was funded by the Instituto de Salud Carlos III (ISCIII) through the project PI21/00588 and co-funded by the European Union
DS Minerva
RD 1 may 2026