RT Journal Article
T1 Blood Transcriptomics of Turbot Scophthalmus maximus: A Tool for Health Monitoring and Disease Studies
A1 Ronza, Paolo
A1 Álvarez Dios, José Antonio
A1 Robledo, Diego
A1 Losada García, Ana Paula
A1 Romero, Roberto
A1 Bermúdez Pose, Roberto
A1 Gómez Pardo, María Belén
A1 Martínez Portela, Paulino
A1 Quiroga Berdeal, María Isabel
K1 RNA-seq
K1 Teleost
K1 Immune system
K1 Immune response
K1 Erythrocytes
K1 Leukocytes
K1 Enteromyxum scophthalmi
K1 Infection
K1 Myxozoa
AB Blood transcriptomics is emerging as a relevant tool to monitor the status of the immune system and assist in diagnosis, prognosis, treatment and pathogenesis studies of diseases. In fish pathology, the potential of transcriptome profiling of blood is still poorly explored. Here, RNA sequencing was applied to analyze the blood transcriptional profile of turbot (Scophthalmus maximus), the most important farmed flatfish. The study was conducted in healthy specimens and specimens parasitized by the myxozoan Enteromyxum scophthalmi, which causes one of the most devastating diseases in turbot aquaculture. The blood of healthy turbot showed a transcriptomic profile mainly related to erythrocyte gas transportation function, but also to antigen processing and presentation. In moderately infected turbot, the blood reflected a broad inhibition of the immune response. Particularly, down-regulation of the B cell receptor signaling pathway was shared with heavily parasitized fish, which showed larger transcriptomic changes, including the activation of the inflammatory response. Turbot response to enteromyxosis proved to be delayed, dysregulated and ineffective in stopping the infection. The study evinces that blood transcriptomics can contribute to a better understanding of the teleost immune system and serve as a reliable tool to investigate the physiopathological status of fish.
PB MDPI
SN 2076-2615
YR 2021
FD 2021-04
LK http://hdl.handle.net/10347/31989
UL http://hdl.handle.net/10347/31989
LA eng
NO Ronza, P.; Álvarez-Dios, J.A.; Robledo, D.; Losada, A.P.; Romero, R.; Bermúdez, R.; Pardo, B.G.; Martínez, P.; Quiroga, M.I. Blood Transcriptomics of Turbot Scophthalmus maximus: A Tool for Health Monitoring and Disease Studies. Animals 2021, 11, 1296. https://doi.org/ani11051296
NO Article with suplementary materials: The following are available online at https://www.mdpi.com/article/10.3390/ani11051296/s1Publicly available datasets were analyzed in this study. Raw sequencing data have been deposited in NCBI’s Short Read Archive (SRA) under BioProject ID PRJNA70378
NO This research was funded by the spanish ministry of economy, industry and competitiveness and the European Regional Development Fund (ERDF), grant number AGL2015–67039–C3–1–R and AGL2015–67039–C3–3–R.The study was conducted in accordance with national (Royal Decree RD53/2013) and European Union (Directive 2010/63/EU) regulation of the on the protection of animals used for scientific purposes and approved by the Ethics Committee of UNIVERDIDADE DE SANTIAGO DE COMPOSTELA (22 November 2016, code: ES1507730055401/16/INV.MED.02/ROD 02)The authors would like to thank Lucía Insua for technical assistance and they acknowledge the support of the Centro de Supercomputación de Galicia (CESGA) in the completion of this work.
DS Minerva
RD 30 abr 2026