RT Journal Article
T1 Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
A1 Carreira Rodríguez, Raquel
A1 Lama Díaz, Tomás
A1 Crugeiras Ríos, María
A1 Aguado Domínguez, Francisco Javier
A1 Sebesta, Marek
A1 Krejci, Lumir
A1 González Blanco, Miguel
K1 D-loop cleavage
K1 Mus81
K1 Yen1
K1 DNA molecules
AB Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements
PB Oxford Academic
SN 0305-1048
YR 2024
FD 2024-07-08
LK http://hdl.handle.net/10347/34913
UL http://hdl.handle.net/10347/34913
LA eng
NO Nucleic Acids Research 52 12 (2024) 7012–7030
NO The work in the Blanco lab was supported by Ministerio de Ciencia e Innovación y Agencia Estatal de Investigación/10.13039/501100011033 [PID2020-115472GB-I00]; Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional ‘Una manera de hacer Europa’ [BFU2016-78121-P]; Xunta de Galicia and Fondo Europeo de Desarrollo Regional ‘Una manera de hacer Europa’ [ED431F- 2016/019, ED431B-2016/016 and ED431C 2019/013]; the work in the Krejci lab was supported by the Czech Science Foundation [21-22593X]; Wellcome Trust collaborative grant [206292/E/17/Z]; R.C., T.L.D., M.C. and F.J.A. were supported by pre-doctoral fellowships from Xunta de Galicia [ED481A-2018/041, ED481A-2018/042, ED481A-2015/011, ED481A 2022/155]; M.C. was also supported by a Collaboration fellowship from ‘Asociación Española Contra el Cáncer’; CIMUS receives financial support from the Xunta de Galicia and Fondo Europeo de Desarrollo Regional [ED431G 2019/02, Centro Singular de Investigación de Galicia, accreditation 2019–2022]. Funding for open access charge: Ministerio de Ciencia e Innovación y Agencia Estatal de Investigación/10.13039/501100011033 [PID2020-115472GB-I00]
DS Minerva
RD 24 abr 2026