RT Journal Article T1 Influence of the storage procedure on the trace element content measured in the aquatic moss Fontinalis antipyretica Hedw A1 Villares Pazos, Rubén A1 Vázquez Castro, María Dolores A1 Real Rodríguez, Carlos K1 Biomonitor K1 Bryophytes K1 Metals K1 River AB Aquatic bryophytes have been used as pollution biomonitors for decades. Despite this, sample collection and preparation methods have not been standardized, which makes it difficult to compare the results of different studies. Most times, the samples have to be stored before processing, for example, when many of them are collected in a short time, as occurs in extensive pollution studies. Storage must be done in a way that does not change the pollutant concentrations in the samples. We studied whether the concentrations of Al, As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, and Zn in the aquatic moss Fontinalis antipyretica were affected by three storage procedures: dry at room temperature, fresh (in refrigerator at 6°C), and frozen at −20°C. In addition, we evaluated whether the subsequent washing of the samples affected the concentrations of these elements differently depending on the storage method. Our results showed that the three methods were, in general, adequate since the concentrations did not change, and we did not observe differences between washed and unwashed samples either. Since the simplest method is refrigeration, we concluded that this is the best of them. However, the concentrations of Hg increased steadily over time in the fresh material, probably because of redistribution after volatilization from the basal parts of the mosses. We believe that the respiration of the plants lowered the concentrations of oxygen inside the hermetically sealed bags containing the samples, thus promoting the reduction of the Hg and its posterior volatilization and redistribution. We did not observe interactions between the storage method and the posterior washing of the samples PB Wiley SN 1061-4303 YR 2023 FD 2023 LK http://hdl.handle.net/10347/30788 UL http://hdl.handle.net/10347/30788 LA eng NO Villares, R., V azquez, M. D., & Real, C. (2023). Influence of the storage procedure on the trace element content measured in the aquatic moss Fontinalis antipyretica Hedw. Water Environment Research, 95(5), e10870. https://doi.org/10.1002/wer.10870 NO Ministerio de Economía y Competitividad(Spain), Grant/Award Number: CTM2015-70578-P; FEDER (EU) DS Minerva RD 24 abr 2026