RT Journal Article
T1 In Vivo Light-Driven DNA Binding and Cellular Uptake of Nucleic Acid Stains
A1 Sánchez López, Mateo Isidro
A1 Martínez Costas, José Manuel
A1 González García, Francisco
A1 Álvarez Bermúdez, María José
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
AB Chemical derivatization of nucleic stains such as ethidium bromide or DAPI with tailored, photoresponsive caging groups, allows for “on demand” spatiotemporal control of their in vivo nucleic acid binding, as well as for improving their cellular uptake. This effect was particularly noteworthy for a nitro-veratryloxycarbonyl-caged derivative of ethidium bromide that, in contrast with the parent stain, is effectively internalized into living cells. The activation strategy works in light-accessible, therapeutically relevant settings, such as human retinas, and can even be applied for the release of active compounds in the eyes of living mice
PB American Chemical Society
SN 1554-8929
YR 2012
FD 2012
LK http://hdl.handle.net/10347/18472
UL http://hdl.handle.net/10347/18472
LA eng
NO Sánchez, M., Martínez-Costas, J., Gonzalez, F., Bermudez, M., Vázquez, M., & Mascareñas, J. (2012). In Vivo Light-Driven DNA Binding and Cellular Uptake of Nucleic Acid Stains. ACS Chemical Biology, 7(7), 1276-1280. doi: 10.1021/cb300100r
NO This document is the Accepted Manuscript version of a Published Work that appeared in final form inACS Chemical Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://pubs.acs.org/doi/10.1021/cb300100r
NO We are thankful for support given by the Spanish grantsSAF2007-61015, SAF2010-20822-C02, CTQ2009-14431/BQU, Consolider Ingenio 2010 CSD2007-00006, and theXunta de Galicia INCITE09 209 084PR, GRC2010/12,PGIDIT08CSA-047209PR. M.I.S. thanks the Spanish Ministryof Education for FPU Ph.D. fellowships
DS Minerva
RD 24 abr 2026