RT Journal Article
T1 Non-synonymous variation and protein structure of candidate genes associated with selection in farm and wild populations of turbot (Scophthalmus maximus)
A1 Andersen, Oivind
A1 Rubiolo Gaytán, Juan Andrés
A1 Pirolli, Davide
A1 Aramburu González, Óscar
A1 Righino, Benedetta
A1 Robledo, Diego
A1 Bouza Fernández, María Carmen
A1 Pampín Iglesias, Marina
A1 Rosa, María Cristina de
A1 Martínez Portela, Paulino
K1 Aquaculture
K1 Circadian rhythms
K1 Genetic variation
K1 Genome
K1 Genotype
K1 Osmoregulation
K1 Protein structure
K1 Scophthalmus maximus
K1 Sequence analysis DNA
AB Non-synonymous variation (NSV) of protein coding genes represents raw material for selection to improve adaptation to the diverse environmental scenarios in wild and livestock populations. Many aquatic species face variations in temperature, salinity and biological factors throughout their distribution range that is reflected by the presence of allelic clines or local adaptation. The turbot (Scophthalmus maximus) is a flatfish of great commercial value with a flourishing aquaculture which has promoted the development of genomic resources. In this study, we developed the first atlas of NSVs in the turbot genome by resequencing 10 individuals from Northeast Atlantic Ocean. More than 50,000 NSVs where detected in the ~ 21,500 coding genes of the turbot genome, and we selected 18 NSVs to be genotyped using a single Mass ARRAY multiplex on 13 wild populations and three turbot farms. We detected signals of divergent selection on several genes related to growth, circadian rhythms, osmoregulation and oxygen binding in the different scenarios evaluated. Furthermore, we explored the impact of NSVs identified on the 3D structure and functional relationship of the correspondent proteins. In summary, our study provides a strategy to identify NSVs in species with consistently annotated and assembled genomes to ascertain their role in adaptation.
PB Nature Research
YR 2023
FD 2023
LK http://hdl.handle.net/10347/32477
UL http://hdl.handle.net/10347/32477
LA eng
NO Andersen, O., Rubiolo, J.A., Pirolli, D., Aramburu, O., Pampín, M., Righino, B., Robledo, D., Bouza, C., De Rosa, M.C. and Martínez, P. (2023). Non-synonymous variation and protein structure of candidate genes associated with selection in farm and wild populations of turbot (Scophthalmus maximus). Scientific reports, 13, 3019. https://doi.org/10.1038/s41598-023-29826-z
NO Tis study was supported by Consellería de Educación, Universidade e Formación Profesional from Xunta deGalicia (Grant No. ED481A2020/119), which additionally supported Oscar Aramburu PhD Tesis with a fellowship (Grant No.: ED481A-2020/119). Te authors wish to thank the provision of DNA samples and population information used in this study of the EU AQUATRACE (No. 311920) project and to the Flanders ResearchInstitute for Agriculture, Fisheries and Food (ILVO, Belgium).
DS Minerva
RD 28 abr 2026