RT Journal Article
T1 Supercritical fluid chromatography-mass spectrometric determination of chiral fungicides in viticulture-related samples
A1 Pérez Mayán, Leticia
A1 Ramil Criado, María
A1 Cela Torrijos, Rafael
A1 Rodríguez Pereiro, Isaac
K1 Fungicides
K1 Enantiomeric fraction
K1 Wine
K1 Soil
K1 Supercritical fluid chromatography
AB Supercritical fluid chromatography (SFC), combined with mass spectrometry (MS), was employed for the determination of five chiral fungicides, from two different chemical families (acylalanine and triazol) in wine and vineyard soils. The effect of different SFC parameters (stationary phase, chiral selector, mobile phase modifier and additive) in the resolution between enantiomers and in the efficiency of compounds ionization at the electrospray source (ESI) was thorougly described. Under final working conditions, chiral separations of selected fungicides were achieved using two different SFC-MS methods, with an analysis time of 10 min and resolution factors from 1.05 to 2.45 between enantiomers. In combination with solid-phase extraction and pressurized liquid extraction, they permitted the enantiomeric determination of target compounds in wine and vineyard soils with limits of quantification in the low ppb range (between 0.5 and 2.5 ng mL−1, and from 1.3 to 6.5 ng g−1, for wine and soil, respectively), and overall recoveries above 80%, calculated using solvent-based standards. For azolic fungicides (tebuconazole, myclobutanil and penconazole) soil dissipation and transfer from vines to wines were non-enantioselective processes. Data obtained for acylalanine compounds confirmed the application of metalaxyl (MET) to vines as racemate and as the R-enantiomer. The enantiomeric fractions (MET-S/(MET-S+MET-R)) of this fungicide in vineyard soils varied from 0.01 to 0.96; moreover, laboratory degradation experiments showed that the relative dissipation rates of MET enantiomers varied depending on the type of soil
PB Elsevier
SN 0021-9673
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27118
UL http://hdl.handle.net/10347/27118
LA eng
NO Journal of Chromatography A 1644 (2021) 462124
NO L.P.M acknowledges a FPU grant to the Spanish Ministry of Science. This study was supported by Xunta de Galicia and Spanish Government through grants GRC-ED431C 2017/36, PGC2018-094613-B-I00, both co-funded by the EU FEDER program
DS Minerva
RD 27 abr 2026