RT Journal Article
T1 Tex10 coordinates epigenetic control of super-enhancer activity in pluripotency and reprogramming
A1 Ding, Junjun
A1 Huang, Xin
A1 Shao, Ningyi
A1 Zhou, Hongwei
A1 Lee, Dung-Fan
A1 Faiola, Francesco
A1 Fidalgo Pérez, Miguel Ángel
A1 Guallar Artal, Diana
A1 Saunders, Arven
A1 Shliaha, Pavel V.
A1 Wang, Hailong
A1 Waghray, Avinash
A1 Papatsenko, Dmitri
A1 Sánchez Priego, Carlos
A1 Li, Dan
A1 Yuan, Ye
A1 Lemischka, Ihor R.
A1 Shen, Li
A1 Kelley, Kevin
A1 Deng, Haiteng
A1 Shen, Xiaohua
A1 Wang, Jianlong
K1 Epigenetics
K1 Super-enhancers
K1 Pluripotency
K1 Reprogramming
AB Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination
PB Cell Press
SN 1934-5909
YR 2015
FD 2015-06-04
LK https://hdl.handle.net/10347/45637
UL https://hdl.handle.net/10347/45637
LA eng
NO Junjun Ding, Xin Huang, Ningyi Shao, Hongwei Zhou, Dung-Fang Lee, Francesco Faiola, Miguel Fidalgo, Diana Guallar, Arven Saunders, Pavel V. Shliaha, Hailong Wang, Avinash Waghray, Dmitri Papatsenko, Carlos Sánchez-Priego, Dan Li, Ye Yuan, Ihor R. Lemischka, Li Shen, Kevin Kelley, Haiteng Deng, Xiaohua Shen, Jianlong Wang, Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming, Cell Stem Cell, Volume 16, Issue 6, 2015, Pages 653-668, ISSN 1934-5909, https://doi.org/10.1016/j.stem.2015.04.001
NO We thank Dr. Jose Silva (United Kingdom) for the pre-iPSCs; Dr. Hitoshi Niwa (Japan) for the 2TS22C ESCs; Dr. Thamar van Dijk (The Nethelands) for Wdr18, Tex10, and Las1L plasmids; and Dr. Huck-Hui Ng (Singapore) for the Nanog-enhancer luciferase construct. Production of the knockout mouse model and injections of siRNAs in early embryos were performed in the Mouse Genetics Shared Resource Facility at the Icahn School of Medicine at Mount Sinai. This research was funded by grants from the NIH to J.W. (1R01-GM095942) and the Empire State Stem Cell Fund through New York State Department of Health (NYSTEM) to J.W. (C028103 and C028121). J.W. is a recipient of an Irma T. Hirschl and Weill-Caulier Trusts Career Scientist Award. A.S. is an awardee of the Traineeship of NIDCR-Interdisciplinary Training in Systems and Developmental Biology and Birth Defects (T32HD075735)
DS Minerva
RD 23 may 2026