RT Journal Article
T1 Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone
A1 Eibes González, Gemma María
A1 Cajthaml, Tomas
A1 Moreira Vilar, María Teresa
A1 Feijoo Costa, Gumersindo
A1 Lema Rodicio, Juan Manuel
K1 Enzymatic degradation
K1 Manganese peroxidase (MnP)
K1 Anthracene
K1 Pyrene
K1 Dibenzothiophene
K1 Miscible solvent
AB The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) greatly hamper their degradation in liquid media. The use of an organic solvent can assist the degradative action of ligninolytic enzymes from white rot fungi. The enzymatic action of the enzyme manganese peroxidase (MnP) in media containing a miscible organic solvent, acetone (36% v/v), was evaluated as a feasible system for the in vitro degradation of three PAHs: anthracene, dibenzothiophene and pyrene. These compounds were degraded to a large extent after a short period of time (7, 24 and 24 h, respectively), at conditions maximizing the MnP-oxidative system. The initial amount of enzyme present in the reaction medium was determinant for the kinetics of the process. The order of degradability, in terms of degradation rates was as follows: anthracene > dibenzothiophene > pyrene. The intermediate compounds were determined using gas chromatography–mass spectrometry and the degradation mechanisms were proposed. Anthracene was degraded to phthalic acid. A ring cleavage product of the oxidation of dibenzothiophene, 4-methoxybenzoic acid, was also observed.
PB Elsevier
SN 0045-6535
SN 1879-1298
YR 2006
FD 2006-06
LK https://hdl.handle.net/10347/39249
UL https://hdl.handle.net/10347/39249
LA eng
NO Gemma Eibes, Tomas Cajthaml, Maria Teresa Moreira, Gumersindo Feijoo, Juan M. Lema, Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone, Chemosphere, Volume 64, Issue 3, 2006, Pages 408-414, ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2005.11.075.
DS Minerva
RD 28 abr 2026