RT Journal Article
T1 First description of adenosine production by Gnomoniopsis smithogilvyi, causal agent of chestnut brown rot
A1 González Jartín, Jesús María
A1 Rodríguez Cañás, Inés
A1 Alvariño Romero, Rebeca
A1 Sáinz Oses, María Jesús
A1 Rodríguez Vieytes, Mercedes
A1 Alfonso Rancaño, María Amparo
A1 Botana López, Luis Miguel
K1 Gnomoniopsis castaneae
K1 Mass spectrometry
K1 Oxasetin
K1 Phytosphingosine
K1 UHPLC-MS-IT-TOF
AB Gnomoniopsis smithogilvyi (Gnomoniaceae, Diaporthales) is the main causal agent of chestnut brown rot on sweet chestnut worldwide. The rotting of nuts leads to alterations in the organoleptic qualities and decreased fruit production, resulting in significant economic losses. In 2021, there was an important outbreak of chestnut rot in southern Galicia (Spanish northwest). The profile of secondary metabolites from G. smithogilvyi was studied, especially to determine its capability for producing mycotoxins, as happens with other rotting fungi, due to the possible consequences on the safety of chestnut consumption. Secondary metabolites produced by isolates of G. smithogilvyi growing in potato dextrose agar (PDA) medium were identified using liquid chromatography coupled with high-resolution mass spectrometry. Three metabolites with interesting pharmacological and phyto-toxicological properties were identified based on their exact mass and fragmentation patterns, namely adenosine, oxasetin, and phytosphingosine. The capacity of G. smithogilvyi to produce adenosine in PDA cultures was assessed, finding concentrations ranging from 176 to 834 µg/kg. Similarly, the production of mycotoxins was ruled out, indicating that the consumption of chestnuts with necrotic lesions does not pose a health risk to the consumer in terms of mycotoxins.
PB Springer
SN 0959-3993
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33671
UL http://hdl.handle.net/10347/33671
LA eng
NO González-Jartín, J.M., Aguín, O., Rodríguez-Cañás, I. et al. First description of adenosine production by Gnomoniopsis smithogilvyi, causal agent of chestnut brown rot. World J Microbiol Biotechnol 40, 148 (2024)
NO The research leading to these results has received funding from the following grants. From Campus Terra (USC), BreveRiesgo (2022-PU011) CLIMIGAL (2022- PU016). From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, GRC (ED431C 2021/01). From Ministerio de Ciencia e Innovación IISCIII/PI19/001248, PID 2020-11262RB-C21, Grant CPP2021-008447 funded by MCIN/AEI/https://doi.org/10.13039/501100011033 and by The European Union NextGenerationEU/PRT. From European Union, Interreg EAPA-0032/2022 – BEAP-MAR, HORIZON-MSCA-2022-DN-01-MSCA Doctoral Networks 2022 101119901-BIOTOXDoc, and HORIZON-CL6-2023-CIRCBIO-01 COMBO-101135438. Authors thank Dirección Xeral de Ordenación e Planificación Forestal (Xunta de Galicia, Spain) and Centro Agroforestal Riós for helpful assistance in the collection of samples.
DS Minerva
RD 30 abr 2026