RT Journal Article
T1 DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization
A1 Gerra, Maria Carla
A1 Carnevali, Davide
A1 Ossola, Paolo
A1 González Villar, Alberto Jacobo
A1 Pedersen, Inge Søkilde
A1 Triñanes, Yolanda
A1 Donnini, Claudia
A1 Manfredini, Matteo
A1 Arendt-Nielsen, Lars
A1 Carrillo de la Peña, María Teresa
K1 Fibromyalgia
K1 Epigenetics
K1 Blood
K1 Biomarkers
K1 DNA methylation
K1 Depression
K1 Immune system
K1 Pain management
AB Fibromyalgia (FM) has been explained as a result of gene-environment interactions. Thepresent study aims to verify DNA methylation differences in eleven candidate genome regionspreviously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers.DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their42 healthy sisters. The associations between the level of methylation in these regions were furtherexplored through a network analysis. Lastly, a logistic regression model investigated the regionspotentially associated with FM, when controlling for sociodemographic variables and depressivesymptoms. The analysis highlighted significant differences in the GCSAML region methylationbetween patients and controls. Moreover, seventeen single CpGs, belonging to other genes, weresignificantly different, however, only one cytosine related to GCSAML survived the correction formultiple comparisons. The network structure of methylation sites was different for each group;GRM2 methylation represented a central node only for FM patients. Logistic regression revealedthat depressive symptoms and DNA methylation in the GRM2 region were significantly associatedwith FM risk. Our study encourages better exploration of GCSAML and GRM2 functions and theirpossible role in FM affecting immune, inflammatory response, and central sensitization of pain
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27516
UL http://hdl.handle.net/10347/27516
LA eng
NO Gerra, M.C.; Carnevali, D.; Ossola, P.; González-Villar, A.; Pedersen, I.S.; Triñanes, Y.; Donnini, C.; Manfredini, M.; Arendt-Nielsen, L.; Carrillo-de-la-Peña, M.T. DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization. J. Clin. Med. 2021, 10, 4992
NO This research was funded by the Spanish Government Funding (Ministerio de Economía y Competitividad: grant PSI2013-45818-R). MCG and LAN are part of the Center for Neuroplasticity and Pain (CNAP) which is supported by the Danish National Research Foundation (DNRF121)
DS Minerva
RD 27 abr 2026