RT Journal Article
T1 Carbon-Coated Superparamagnetic Nanoflowers for Biosensors Based on Lateral Flow Immunoassays
A1 Moyano, Amanda
A1 Serrano Pertierra, Esther
A1 Salvador, María
A1 Martínez García, José Carlos
A1 Piñeiro Redondo, Yolanda
A1 Yáñez Vilar, Susana
A1 González Gómez, Manuel Antonio
A1 Rivas Rey, José
A1 Rivas, Montserrat
A1 Blanco López, M. Carmen
K1 Superparamagnetic iron oxide nanoflowers
K1 Lateral flow immunoassays
K1 Biosensor
K1 Extracellular vesicles
K1 Exosomes
AB Superparamagnetic iron oxide nanoflowers coated by a black carbon layer (Fe3O4@C) were studied as labels in lateral flow immunoassays. They were synthesized by a one-pot solvothermal route, and they were characterized (size, morphology, chemical composition, and magnetic properties). They consist of several superparamagnetic cores embedded in a carbon coating holding carboxylic groups adequate for bioconjugation. Their multi-core structure is especially efficient for magnetic separation while keeping suitable magnetic properties and appropriate size for immunoassay reporters. Their functionality was tested with a model system based on the biotin–neutravidin interaction. For this, the nanoparticles were conjugated to neutravidin using the carbodiimide chemistry, and the lateral flow immunoassay was carried out with a biotin test line. Quantification was achieved with both an inductive magnetic sensor and a reflectance reader. In order to further investigate the quantifying capacity of the Fe3O4@C nanoflowers, the magnetic lateral flow immunoassay was tested as a detection system for extracellular vesicles (EVs), a novel source of biomarkers with interest for liquid biopsy. A clear correlation between the extracellular vesicle concentration and the signal proved the potential of the nanoflowers as quantifying labels. The limit of detection in a rapid test for EVs was lower than the values reported before for other magnetic nanoparticle labels in the working range 0–3 × 107 EVs/μL. The method showed a reproducibility (RSD) of 3% (n = 3). The lateral flow immunoassay (LFIA) rapid test developed in this work yielded to satisfactory results for EVs quantification by using a precipitation kit and also directly in plasma samples. Besides, these Fe3O4@C nanoparticles are easy to concentrate by means of a magnet, and this feature makes them promising candidates to further reduce the limit of detection
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23662
UL http://hdl.handle.net/10347/23662
LA eng
NO Moyano, A.; Serrano-Pertierra, E.; Salvador, M.; Martínez-García, J.C.; Piñeiro, Y.; Yañez-Vilar, S.; Gónzalez-Gómez, M.; Rivas, J.; Rivas, M.; Blanco-López, M.C. Carbon-Coated Superparamagnetic Nanoflowers for Biosensors Based on Lateral Flow Immunoassays. Biosensors 2020, 10, 80
NO This work was supported in part by Spanish Ministry of Economy and Competitiveness under projects MAT2017-84959-C2-1-R and the Principality of Asturias (Spain) under project IDI/2018/000185 and the Consellería de Educación Program for Development of a Strategic Grouping in Materials (AEMAT) at the University of Santiago de Compostela under Grant No. ED431E208/08, Xunta de Galicia. Amanda Moyano was supported by a “Severo Ochoa” fellowship (Consejería de Educación y Cultura del Gobierno del Principado de Asturias, grant BP17-152)
DS Minerva
RD 25 abr 2026