RT Journal Article
T1 Chemical sensors for the early diagnosis of bacterial resistance to β-lactam antibiotics
A1 Canabal Falcón, Rafael
A1 González Bello, Concepción
K1 Antibiotic resistance detection
K1 Colorimetric sensors
K1 Fluorescent sensors
K1 Carbapenemases
K1 Enzyme substrates
AB β-Lactamases are bacterial enzymes that inactivate β-lactam antibiotics and, as such, are the most prevalent cause of antibiotic resistance in Gram-negative bacteria. The ever-increasing production and worldwide dissemination of bacterial strains producing carbapenemases is currently a global health concern. These enzymes catalyze the hydrolysis of carbapenems – the β-lactam antibiotics with the broadest spectrum of activity that are often considered as drugs of last resort. The incidence of carbapenem-resistant pathogens such as Pseudomonas aeruginosa, Acinetobacter baumannii and carbapenemase or extended spectrum beta-lactamase (ESBL)-producing Enterobacterales, which are frequent in clinical settings, is worrisome since, in some cases, no therapies are available. These include all metallo-β-lactamases (VIM, IMP, NDM, SMP, and L1), and serine-carbapenemases of classes A (KPC, SME, IMI, and GES), and of classes D (OXA-23, OXA-24/40, OXA-48 and OXA-58). Consequently, the early diagnosis of bacterial strains harboring carbapenemases is a pivotal task in clinical microbiology in order to track antibiotic bacterial resistance and to improve the worldwide management of infectious diseases. Recent research efforts on the development of chromogenic and fluorescent chemical sensors for the specific and sensitive detection and quantification of β-lactamase production in multidrug-resistant pathogens are summarized herein. Studies to circumvent the main limitations of the phenotypic and molecular methods are discussed. Recently reported chromogenic and fluorogenic cephalosporin- and carbapenem-based β-lactamase substrates will be reviewed as alternative options to the currently available nitrocefin and related compounds, a chromogenic cephalosporin-based reagent widely used in clinical microbiology laboratories. The scope of these new chemical sensors, along with the synthetic approaches to synthesize them, is also summarized
PB Elsevier
SN 0045-2068
YR 2024
FD 2024-06-04
LK http://hdl.handle.net/10347/34968
UL http://hdl.handle.net/10347/34968
LA eng
NO Bioorganic Chemistry 150 (2024) 107528
NO This work was funded by the Spanish State Agency of Research (PID2022-136963OB I00/AEI/10.13039/501100011033, CG-B), the Xunta de Galicia [ED431C 2021/29 and Centro singular de investigación de Galicia accreditation 2023-2027 (ED431G 2023/03), CG-B], and the European Regional Development Fund (ERDF). We thank Dr. Jorge Arca-Suárez for helpful discussions on writing this manuscript
DS Minerva
RD 30 abr 2026