RT Journal Article
T1 Impact of Different Groups of Active Substances for Fungal Control on Vineyard Soil Microbiota and Pesticide Residue Profiles
A1 Loureiro-Rodríguez, M. Dolores
A1 Rodríguez Pereiro, Isaac
A1 Fernández Fernández, Victoria
A1 Ramil Criado, María
A1 Díaz-Losada, Emilia
K1 Active substance
K1 Azoxystrobin
K1 Bacteria
K1 Fungi
K1 Metrafenone
K1 Pesticide pollution
K1 Soil diversity
K1 Vineyard
AB Pesticide use in agriculture can have negative collateral effects on the environment. In this study, two groups of treatments (G1 and G2) based on active substances (ASs) with different mobility were evaluated in order to determine pesticide residues in the soil and their impact on soil microbial populations in two vineyards located in two Denominations of Origin (D.O.). Soil samples were collected in July, October, and the following March over two consecutive years. Pesticide residues were analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) after QuEChERS extraction. Microbial genera were identified by the amplification of the fungal ITS regions with the universal primers ITS86F and ITS4R, and the bacterial 16S rRNA gene (V4 region) with primers 515F and 806R. Although G1 consistently showed higher residues, primarily attributable to azoxystrobin, no significant differences were observed between the two pesticide groups in the total pesticide residues or diversity of microbial communities. The factors D.O., campaign, and sampling month influenced the concentration of residues. Several ASs exhibited different dissipation dynamics depending on the D.O. Azoxystrobin and metrafenone were the most persistent in soil. The LEfSe analysis associated four beneficial fungal genera with the G2 group. The judicious selection of ASs can help to minimize the pesticide residues in soil and their harmful effects on beneficial genera.
PB MDPI
YR 2026
FD 2026
LK https://hdl.handle.net/10347/45652
UL https://hdl.handle.net/10347/45652
LA eng
NO Loureiro-Rodríguez, M. D., Graña-Caneiro, M. J., Vázquez-Arias, A., Abarquero, E., Rodríguez, I., Fernández-Fernández, V., Ramil, M., Štůsková, K., Frejlichová, L., Rodríguez-Cruz, M. S., Marín-Benito, J. M., & Díaz-Losada, E. (2026). Impact of Different Groups of Active Substances for Fungal Control on Vineyard Soil Microbiota and Pesticide Residue Profiles. Agriculture, 16(3), 344. https://doi.org/10.3390/agriculture16030344
NO This research is part of the project TED2021–129962B-C43, funded by the Spanish Ministry of Science and Innovation (MICIU/AEI/10.13039/501100011033), and by the European Union NextGeneration EU/PRTR. Ester Abarquero is grateful for her predoctoral contract (ref. INIA-2020-0012) granted by MICIU/AEI/10.13039/501100011033 and ESF Investing in your future. Anxo Vázquez-Arias is grateful for his predoctoral contract (ref. PRE2022-103198) funded by MICIU/AEI/10.13039/501100011033 and European Social Fund Plus (ESF+). María Dolores Loureiro-Rodríguez gratefully acknowledges the Xunta de Galicia, which, under Resolution of the Axencia Galega de Innovación of 29 July 2021, granted her a Senior Talent Program contract. This research used the infrastructure acquired by project CZ.02.1.01/0.0/0.0/16_017/0002334 Research Infrastructure for Young Scientists, which is co-financed by the Operational Program of Research, Development and Education.
DS Minerva
RD 24 abr 2026