RT Journal Article
T1 Detection of Anatoxin-a and Three Analogs in Anabaena spp. Cultures: New Fluorescence Polarization Assay and Toxin Profile by LC-MS/MS
A1 Sánchez Salguero, Jon Andoni
A1 Otero Fuertes, María Paz
A1 Alfonso Rancaño, María Amparo
A1 Ramos, Vitor
A1 Vasconcelos, Vitor
A1 Aráoz, Romulo
A1 Molgó, Jordi
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Anatoxin-a
K1 Nicotinic acetylcholine receptor
K1 Fluorescence polarization
K1 Liquid chromatography-mass spectrometry
K1 Anabaena spp.
AB Anatoxin-a (ATX) is a potent neurotoxin produced by several species of Anabaena spp. Cyanobacteria blooms around the world have been increasing in recent years; therefore, it is urgent to develop sensitive techniques that unequivocally confirm the presence of these toxins in fresh water and cyanobacterial samples. In addition, the identification of different ATX analogues is essential to later determine its toxicity. In this paper we designed a fluorescent polarization (FP) method to detect ATXs in water samples. A nicotinic acetylcholine receptor (nAChR) labeled with a fluorescein derivative was used to develop this assay. Data showed a direct relationship between the amount of toxin in a sample and the changes in the polarization degree of the emitted light by the labeled nAChR, indicating an interaction between the two molecules. This method was used to measure the amount of ATX in three Anabaena spp. cultures. Results indicate that it is a good method to show ATXs presence in algal samples. In order to check the toxin profile of Anabaena cultures a LC-MS/MS method was also developed. Within this new method, ATX-a, retention time (RT) 5 min, and three other molecules with a mass m/z 180.1 eluting at 4.14 min, 5.90 min and 7.14 min with MS/MS spectra characteristic of ATX toxin group not previously identified were detected in the Anabaena spp. cultures. These ATX analogues may have an important role in the toxicity of the sample
PB MDPI
YR 2014
FD 2014
LK http://hdl.handle.net/10347/23590
UL http://hdl.handle.net/10347/23590
LA eng
NO Sanchez, J.A.; Otero, P.; Alfonso, A.; Ramos, V.; Vasconcelos, V.; Aráoz, R.; Molgó, J.; Vieytes, M.R.; Botana, L.M. Detection of Anatoxin-a and Three Analogs in Anabaena spp. Cultures: New Fluorescence Polarization Assay and Toxin Profile by LC-MS/MS. Toxins 2014, 6, 402-415
NO The research leading to these results has received funding from the following FEDER cofunded-grants: From Ministerio de Ciencia y Tecnología, Spain: AGL2009-13581-CO2-01, AGL2012-40485-CO2-01. From Xunta de Galicia, Spain: 10PXIB261254 PR.From the European Union’s Seventh Framework Programme managed by REA—Research Executive Agency http://ec.europa.eu/research/rea (FP7/2007-2013) under grant agreement Nos. 211326—CP (CONffIDENCE), 265896 BAMMBO, 265409 µAQUA, and 262649 BEADS, 315285 Ciguatools and 312184 PharmaSea. From the Atlantic Area Programme (Interreg IVB Trans-national): 2008-1/003 (Atlantox) and 2009-1/117 (Pharmatlantic)
DS Minerva
RD 24 abr 2026