RT Journal Article
T1 Hydrazone-modulated peptides for efficient gene transfection
A1 Louzao Pernas, Iria
A1 García Fandiño, Rebeca
A1 Montenegro García, Javier
K1 Gene transfection
K1 Hydrazone-modulated peptides
AB Gene transfection continues to be a major challenge in chemistry, biology and materials sciences. New methodologies and recent breakthroughs have renewed the interest in the discovery and development of new tools for efficient gene transfection. Hydrazone formation between a cationic head and hydrophobic tails has emerged as one of the most promising techniques for nucleotide delivery. In this contribution, we have exploited hydrazone formation to modulate the transfection activity of a parent linear peptide in combination with a plasmid DNA cargo. This strategy allowed the straightforward preparation, under physiologically compatible conditions, of a discrete library of amphiphilic modulated penetrating peptides. Without the requirement of any isolation or purification steps, these modulated amphiphilic peptides were combined with a plasmid DNA and screened in transfection experiments of human HeLa cells. Three of these hydrazone-conjugated peptides were identified as excellent vectors for plasmid delivery with comparable, or even higher, efficiencies and lower toxicity than the commercial reagents employed in routine transfection assays
PB Royal Society of Chemistry
SN 2050-750X
YR 2017
FD 2017-02-22
LK http://hdl.handle.net/10347/15266
UL http://hdl.handle.net/10347/15266
LA eng
NO Louzao, I., García-Fandiño, R. and Montenegro, J. (2017), Hydrazone-modulated peptides for efficient gene transfection. J. Mater. Chem. B. [doi: 10.1039/c7tb00179g]. Royal Society of Chemistry
NO We are thankful to Dr. Irene Lostalé-Seijo for cell culture assistance and discussions. We acknowledge funding from the Spanish Government MINECO: [CTQ2014-59646-R] and [CTQ2015-74621-JIN], the Xunta de Galicia (ED431G/09), the ERDF and the CESGA. R. G.-F. received a FCT Investigator Grant from Portugal (IF/01133/2015). J.M. received a Ramon y Cajal (RYC-2013-13784) and an ERC Starting Investigator Grant (DYNAP-677786)
DS Minerva
RD 29 abr 2026