RT Journal Article
T1 A detailed proteomic analysis of rhodocytin-activated platelets reveals novel clues on the CLEC-2 signalosome: implications for CLEC-2 signaling regulation
A1 Parguiña, Andrés F.
A1 Alonso, Jana
A1 Rosa, Isaac
A1 Vélez, Paula
A1 González-López, María J.
A1 Guitián, Esteban
A1 Eble, Johannes A.
A1 Loza, María I.
A1 García, Ángel
K1 Blood platelets
K1 Signal transduction
K1 Tyrosine
K1 Phosphotyrosine
K1 Adap trial
K1 Proteome
K1 Ships
K1 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
AB C-type lectin-like receptor 2 (CLEC-2) is an essential platelet-activating receptor in hemostasis and thrombosis that is activated by the snake venom rhodocytin. We present here a differential proteomic analysis of basal and rhodocytin-activated platelets with the aim of providing novel clues on CLEC-2 signaling regulation. Proteome analysis was based on 2D-DIGE, phosphotyrosine immunoprecipitations followed by 1D SDS-PAGE and mass spectrometry. Protein-protein interactions were studied by coimmunoprecipitations and a systems biology approach. Overall, we identified 132 proteins differentially regulated after CLEC-2 platelet activation, including most of the major players reported so far in the signaling cascade. In addition, we identified various proteins not previously known to participate in CLEC-2 signaling, such as the adapters Dok-2 and ADAP, tyrosine kinase Fer, and tyrosine phosphatase SHIP-1. We also report an increased association between Dok-2 and SHIP-1 in rhodocytin-stimulated platelets, which might negatively regulate CLEC-2 signaling. Moreover, we also present a comparative analysis of proteomic data for CLEC-2 and glycoprotein VI signaling. We think that our data provide thrombosis-relevant information on CLEC-2 signaling regulation, contributing to a better understanding of this important signaling cascade.
PB American Society of Hematology (ASH Publications)
SN 0006-4971
YR 2012
FD 2012-12-20
LK https://hdl.handle.net/10347/44592
UL https://hdl.handle.net/10347/44592
LA eng
NO Parguiña, A.F., Alonso, J., Rosa, I., Vélez, P., González-López, M.J.,Guitián, E.,Eble, J.A., Loza, M.I., García, A. (2012). A detailed proteomic analysis of rhodocytin-activated platelets reveals novel clues on the CLEC-2 signalosome: implications for CLEC-2 signaling regulation. "Blood", vol. 120, 26
NO Xunta de Galicia
NO MINECO
DS Minerva
RD 24 abr 2026