RT Journal Article
T1 Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica
A1 Villares Pazos, Rubén
A1 Real Rodríguez, Carlos
A1 Vázquez Castro, María Dolores
K1 Chlorophyll
K1 Pheophytin
K1 Pigment ratios
K1 Bioindicators
K1 Bryophytes
K1 Rivers
AB Aquatic bryophytes are frequently used as bioindicators of water quality in rivers. Poor water quality increases physiological stress in moss, and stress levels can be estimated by measuring the concentrations of photosynthetic pigments and by calculating pigmentary indices. However, studies collecting many samples in a short time may need to store them until analysis. In the meantime, the pigments may suffer some degradation. Given the lack of studies on this problem for aquatic bryophytes, this one investigates the effect of three storage methods (refrigerated at 6 °C, frozen at − 20 °C and dried at room temperature) on the concentrations of chlorophyll a, chlorophyll b, pheophytin a and the following ratios: chlorophyll a/chlorophyll b, D430/D410, D665/D665a, D430/D665 and D480/D665 (where Dx is the absorbance at x nm) in the aquatic moss Fontinalis antipyretica. The results showed that refrigeration was the most suitable method, freezing might be suitable for some parameters, and drying was inadequate for this purpose. We recommend that all studies on photosynthetic pigments detail the time elapsed from collection to sample analysis, as well as the storage method used.
PB Springer
SN 0018-8158
YR 2024
FD 2024
LK http://hdl.handle.net/10347/34720
UL http://hdl.handle.net/10347/34720
LA eng
NO Villares, R., Real, C. & Vázquez, M.D. Influence of storage method on the content of photosynthetic pigments of the aquatic moss Fontinalis antipyretica. Hydrobiologia 851, 4167–4176 (2024). https://doi.org/10.1007/s10750-024-05573-8
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by Ministerio de Economía y Competitividad (Spain), project CTM2015-70578-P. The authors belong to the Galician Competitive Research Group GRC/GPC2016-002. These programmes are co-funded by FEDER (EU).
DS Minerva
RD 29 abr 2026