RT Journal Article
T1 Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice
A1 Rojo Montejo, Silvia
A1 Collantes Fernández, Esther
A1 Rodríguez Marcos, Sonia
A1 Pérez Zaballos, Francisco
A1 López Pérez, Inmaculada
A1 Prenafeta, Antoni
A1 Ortega Mora, Luis Miguel
K1 Neospora caninum
K1 Inactivated vaccine
K1 Tachyzoite
K1 Bradyzoite
K1 Mice
AB The worldwide economic impact of Neospora caninum infection has caused the development of effective vaccines to become one of the main goals in the field of neosporosis research. In this study, the protection conferred by antigens from inactivated whole tachyzoites (TZ) and a tachyzoite-bradyzoite mixture (TZ-BZ) of N. caninum (Nc-Spain7 isolate) incorporated into a water-in-oil emulsion (W/O) and aluminium hydroxide-ginseng extract (Al/G) was evaluated in mouse models of congenital and cerebral N. caninum infection. Immunization with TZ-BZ induced congenital and cerebral neosporosis exacerbation that was mainly characterized by reduced neonatal median survival time and increased parasite presence in adult mouse brains. The immune response of mice immunized with TZ-BZ was characterized by an increase in IFN-γ expression prior to challenge and an increase in IL-4 expression accompanied with significantly higher levels of antibodies against 2 recombinant bradyzoite-specific proteins (rNcSAG4 and rNcBSR4) after challenge. Immunization with TZ in W/O significantly reduced neonatal mortality, vertical transmission as well as parasite presence in adult mouse brains and induced a strong humoral immune response. The current study demonstrates the critical role of stage-specific antigens and adjuvants on the development of effective inactivated vaccines for the prevention of N. caninum infection.
PB Cambridge University Press
SN 0031-1820
YR 2011
FD 2011
LK https://hdl.handle.net/10347/39494
UL https://hdl.handle.net/10347/39494
LA eng
NO ROJO-MONTEJO S, COLLANTES-FERNÁNDEZ E, RODRÍGUEZ-MARCOS S, et al. Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice. Parasitology. 2011;138(11):1372-1383. doi:10.1017/S0031182011001156
NO This work was supported by the PETRI 95-0777.OP project from the Spanish Government and HIPRA.
DS Minerva
RD 24 abr 2026