RT Journal Article T1 Subgingival microbiota in health compared to periodontitis and the influence of smoking A1 Camelo Castillo, Anny J. A1 Mira, Alex A1 Pico Blanco, Alexandre A1 Nibali, Luigi A1 Henderson, Brian A1 Donos, Nikolaos A1 Tomás Carmona, Inmaculada K1 16S ribosomal RNA K1 Chroni periodontitis K1 Dental plaque K1 Microbiome K1 Oral health K1 Smoking AB Background: Age determination has great importance in many clinical decisions, being commonly used inodontopediatrics, orthodontics, pediatrics, and forensic medicine. The Nolla and Demirjian et al. methods havebeen used for these purposes. However, estimating chronological age by means of the dental mineralization stageis not a straightforward analysis, and it is fundamental to test the validity of these methods and their applicability topopulations. In this article we intend to compare the accuracy of estimating chronological age from dental agemeasured with the Nolla and Demirjian methods in a Portuguese and Spanish sample, considering the variables ofsex and age-group.Methods: The sample was composed of 821 orthopantomographs of healthy Portuguese (n = 270) and Spanish(n = 551) subjects from 4 to 34 years old. For the Nolla and Demirjian methods, seven mandibular left teeth wereexamined, staged according to the dental maturity scale of each method. We obtained a good index of inter-rateragreement, a good internal consistency for the teeth assessment, and a good temporal consistency.Results: Dental age was calculated for each method. The Demirjian et al. method tends to overestimate the realage of participants and the Nolla method tends to underestimate it. The accuracy of both methods varied betweenthe sexes and age groups. Both methods were found to be more precise with males. As the age-group increases,the predictive capacities of both methods diminish. The Nolla method was more accurate than the Demirjianmethod in early and late childhood for both sexes. Neither method could predict chronological age in adults.Conclusions: We can estimate chronological age for early and late childhood, through the Nolla and Demirjianmethods, with the former showing greater predictive capacities than the latter. The Demirjian method tends tooverestimate age and the Nolla method tends to underestimate it, leading to the importance of forming regressionequations adapted to the population studied. Nolla and Demirjian formulas adapted to our sample were created asa function of sex and age-group. PB Frontiers Media YR 2015 FD 2015 LK http://hdl.handle.net/10347/23030 UL http://hdl.handle.net/10347/23030 LA eng NO Camelo-Castillo AJ, Mira A, Pico A, Nibali L, Henderson B, Donos N and Tomás I (2015) Subgingival microbiota in health compared to periodontitis and the influence of smoking. Front. Microbiol. 6:119. doi: 10.3389/fmicb.2015.00119 DS Minerva RD 28 abr 2026