RT Journal Article
T1 Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs
A1 Folgueira López, Iria
A1 Noia Guldrís, Manuel
A1 Blanco Abad, Verónica
A1 Mallo Seijas, Natalia
A1 Leiro Vidal, José Manuel
A1 Lamas Fernández, Jesús
K1 Fish
K1 Peritoneum
K1 Adjuvant
K1 Microparticles
K1 Phagocytes
K1 Migration
AB New adjuvants based on microparticles are being developed for use in fish vaccines. The size of the microparticles may affect the immune response generated, as the adjuvant can either be retained at the site of injection or transported to lymphoid organs. The objectives of this study were to evaluate the maximum size of particles that can be exported out of the cavity, to determine the phagocytosis kinetics and to establish the routes whereby particle-containing cells move from the peritoneal cavity after injection. Fish were injected intraperitoneally with fluorescent cyclodextrins or with fluorescent particles of different size (0.1–10 μm). Phagocytes containing beads of size 4 μm or larger did not reach lymphoid organs, although some were able to cross the peritoneal mesothelium. The number of free peritoneal neutrophils and macrophage-like cells containing beads peaked at 6 and 24 h respectively, and the numbers then decreased quickly, indicating migration of cells to the peritoneum or other body areas. Migration of cells containing beads mainly occurs through the visceral peritoneum. These cells were found on the latero-ventral surfaces of the peritoneal folds that connect the visceral organs. Except for some vascularised areas, the surfaces of liver, stomach and intestine were devoid of particle-containing cells. Some cells containing beads were also found attached to the parietal peritoneum, although in lower numbers than in the visceral peritoneum. Such cells were also found in high numbers in the spleen and kidney 6 h post injection. Because cells containing phagocytosed material quickly become attached to the peritoneum or migrate to lymphoid organs, the immune response generated by a vaccine or by an inflammatory stimulus should probably be evaluated in attached cells as well as in free peritoneal cells.
PB Elsevier
SN 1050-4648
YR 2015
FD 2015-04-01
LK https://hdl.handle.net/10347/45959
UL https://hdl.handle.net/10347/45959
LA eng
NO Folgueira, I., Noia, M., Blanco-Abad, V., Mallo, N., Leiro, J., & Lamas, J. (2015). Particle size and traffic of phagocytes between the turbot peritoneal cavity and lymphoid organs. Fish & Shellfish Immunology, 44(2), 652–661. doi:10.1016/j.fsi.2015.03.034
DS Minerva
RD 24 abr 2026