RT Journal Article
T1 Characterization of Foodborne Strains of Staphylococcus aureus by Shotgun Proteomics: Functional Networks, Virulence Factors and Species-Specific Peptide Biomarkers
A1 Carrera, Mónica
A1 Böhme, Karola
A1 Gallardo, José M.
A1 Barros Velázquez, Jorge
A1 Cañas, Benito
A1 Calo Mata, María Pilar
K1 Foodborne
K1 Staphylococcus aureus
K1 Shotgun proteomics
K1 Mass spectrometry
K1 Functional networks
K1 Virulence factors
K1 Peptide biomarkers
AB In the present work, we applied a shotgun proteomics approach for the fast and easy characterization of 20 different foodborne strains of Staphylococcus aureus (S. aureus), one of the most recognized foodborne pathogenic bacteria. A total of 644 non-redundant proteins were identified and analyzed via an easy and rapid protein sample preparation procedure. The results allowed the differentiation of several proteome datasets from the different strains (common, accessory, and unique datasets), which were used to determine relevant functional pathways and differentiate the strains into different Euclidean hierarchical clusters. Moreover, a predicted protein-protein interaction network of the foodborne S. aureus strains was created. The whole confidence network contains 77 nodes and 769 interactions. Most of the identified proteins were surface-associated proteins that were related to pathways and networks of energy, lipid metabolism and virulence. Twenty-seven virulence factors were identified, and most of them corresponded to autolysins, N-acetylmuramoyl-L-alanine amidases, phenol-soluble modulins, extracellular fibrinogen-binding proteins and virulence factor EsxA. Potential species-specific peptide biomarkers were screened. Twenty-one species-specific peptide biomarkers, belonging to eight different proteins (nickel-ABC transporter, N-acetylmuramoyl-L-alanine amidase, autolysin, clumping factor A, gram-positive signal peptide YSIRK, cysteine protease/staphopain, transcriptional regulator MarR, and transcriptional regulator Sar-A), were proposed to identify S. aureus. These results constitute the first major dataset of peptides and proteins of foodborne S. aureus strains. This repository may be useful for further studies, for the development of new therapeutic treatments for S. aureus food intoxications and for microbial source-tracking in foodstuffs
PB Frontiers Media
YR 2017
FD 2017
LK http://hdl.handle.net/10347/21565
UL http://hdl.handle.net/10347/21565
LA eng
NO Carrera M, Böhme K, Gallardo JM, Barros-Velázquez J, Cañas B and Calo-Mata P (2017) Characterization of Foodborne Strains of Staphylococcus aureus by Shotgun Proteomics: Functional Networks, Virulence Factors and Species-Specific Peptide Biomarkers. Front. Microbiol. 8:2458. doi: 10.3389/fmicb.2017.02458
NO This work was funded by the Spanish Ministry of Economy and Competitivity Project AGL2013-48244-R and by the European Regional Development Fund (2007–2013). The authors thank Drs. Morandi and Brasca at the Institute of Sciences of Food Production (ISPA-CNR) in Milan, Italy, and Drs. Cremonesi and Castiglioni at the Institute of Agricultural Biology and Biotechnology (IBBA-CNR) in Milan, Italy, for their support in the collection of the S. aureus strains used in the present study
DS Minerva
RD 24 abr 2026