RT Journal Article
T1 Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer
A1 Herrero Filgueira, Carolina
A1 Fuente González, Alexandre de la
A1 Casas Arozamena, Carlos
A1 Sebastián, Víctor
A1 Prieto, Martín
A1 Arruebo, Manuel
A1 Abalo, Alicia
A1 Colás, Eva
A1 Moreno Bueno, Gema
A1 Gil Moreno, Antonio
A1 Vilar, Ana
A1 Cueva, Juan
A1 Abal Posada, Miguel
A1 Muinelo Romay, Laura
K1 EVs
K1 ExoGAG
K1 Endometrial cancer
K1 ANXA2
AB Tumor-derived extracellular vesicles (EVs) are secreted in large amounts into biological fluids of cancer patients. The analysis of EVs cargoes has been associated with patient´s outcome and response to therapy. However, current technologies for EVs isolation are tedious and low cost-efficient for routine clinical implementation. To explore the clinical value of circulating EVs analysis we attempted a proof-of-concept in endometrial cancer (EC) with ExoGAG, an easy to use and highly efficient new technology to enrich EVs. Technical performance was first evaluated using EVs secreted by Hec1A cells. Then, the clinical value of this strategy was questioned by analyzing the levels of two well-known tissue biomarkers in EC, L1 cell adhesion molecule (L1CAM) and Annexin A2 (ANXA2), in EVs purified from plasma in a cohort of 41 EC patients and 20 healthy controls. The results demonstrated the specific content of ANXA2 in the purified EVs fraction, with an accurate sensitivity and specificity for EC diagnosis. Importantly, high ANXA2 levels in circulating EVs were associated with high risk of recurrence and non-endometrioid histology suggesting a potential value as a prognostic biomarker in EC. These results also confirmed ExoGAG technology as a robust technique for the clinical implementation of circulating EVs analyses
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/23863
UL http://hdl.handle.net/10347/23863
LA eng
NO Herrero, C.; de la Fuente, A.; Casas-Arozamena, C.; Sebastian, V.; Prieto, M.; Arruebo, M.; Abalo, A.; Colás, E.; Moreno-Bueno, G.; Gil-Moreno, A.; Vilar, A.; Cueva, J.; Abal, M.; Muinelo-Romay, L. Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer. Cancers 2019, 11, 2000
NO This research was funded by Instituto de Salud Carlos III, grant PI17/01919, co-financed by the European Regional Development Fund (FEDER), and by Fundación Científica de la Asociación Española Contra el Cáncer (AECC), Grupos Clínicos Coordinados 2018. Carolina Herrero is supported by a predoctoral i-PFIS fellowship from Instituto de Salud Carlos III (IFI17/00047); Laura Muinelo is supported by Asociación Española Contra el Cáncer (AECC)
DS Minerva
RD 23 abr 2026