RT Journal Article
T1 Where in the cell is our cargo? Current methods to study intracellular cytosolic localization
A1 Méndez Ardoy, Alejandro
A1 Lostalé Seijo, Irene
A1 Montenegro García, Javier
K1 Proteins
K1 Drug delivery
K1 Fluorophores
K1 Microscopy
K1 Cells
AB The internalization and delivery of active substances into cells is a field of growing interest for chemical biology and therapeutics. As we move from small‐molecule based drugs towards bigger cargos, such as antibodies, enzymes, nucleases or nucleic acids, the development of efficient delivery systems becomes critical for their practical application. Different strategies and synthetic carriers have been developed including cationic lipids, gold nanoparticles, polymers, cell‐penetrating peptides, protein surface modification, etc. However, all these methodologies still present limitations related to the precise targeting of the different intracellular compartments and, in particular, the difficult access to the cellular cytosol. Additionally, the precise quantification of the cellular uptake of a molecule is not enough to demonstrate delivery and/or functional activity. Therefore, methods to determine the cellular distribution of cargos and carriers are of critical importance to identify the barriers that are blocking the activity. In this mini‐review, we survey the different techniques that can be currently used to track and monitor the subcellular localization of the synthetic molecules that we deliver inside cells
YR 2018
FD 2018-09-03
LK http://hdl.handle.net/10347/17307
UL http://hdl.handle.net/10347/17307
LA eng
NO Méndez Ardoy, A., Lostalé-Seijo, I., & Montenegro, J. (2018). Where in the cell is our cargo? Current methods to study intracellular cytosolic localization. Chembiochem. doi: 10.1002/cbic.201800390
NO NOTICE: This is the Accepted Version of the following article: Méndez Ardoy, A., Lostalé-Seijo, I., & Montenegro, J. (2018). Where in thecell is our cargo? Current methods to study intracellular cytosolic localization. Chembiochem. doi: 10.1002/cbic.201800390© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
NO We  acknowledge  funding  from  the  Spanish  Agencia Estatal  de Investigación (AEI) [CTQ2014-59646-R, SAF2017-89890-R], the Xunta  de  Galicia  (ED431G/09,  ED431C  2017/25  and  2016-AD031) and the ERDF. A. M.-A.  received a MCIF from the EC (GLYCONANOPEP-750248).  J.  M.  received  a  Ramón  y  Cajal (RYC-2013-13784),  an  ERC  Starting  Investigator  Grant (DYNAP-677786)  and  a  Young  Investigator  Grant  from  the Human Frontier Science Research Program (RGY0066/2017)
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RD 24 abr 2026