RT Journal Article T1 Characterization of active films of chitosan containing nettle Urtica dioica L. extract: Spectral and water properties, microstructure, and antioxidant activity A1 Flórez, María A1 Cazón Díaz, Patricia A1 Vázquez Vázquez, Manuel K1 Nettle extract K1 Chitosan K1 Active packaging AB Chitosan films enriched with aqueous nettle extract (Urtica dioica L.) were evaluated by measuring their solubility, equilibrium moisture, water vapor permeability, spectral and antioxidant properties, and microstructure. Nettle extract showed a significant effect on the analyzed film properties. The addition of nettle extract manifested a sharp decrease in water vapor permeability, decreasing from 5.64 · 10−11 to 2.22 · 10−11 g/m·s·Pa. The chitosan- nettle extract films exhibited a high free-radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Incorporation of nettle extract into the chitosan matrix was successfully carried out to obtain antioxidant films. The results obtained showed that the incorporation of nettle extract allowed obtaining chitosan films with antioxidant properties, including a total phenolic content up to 1.57 mg GAE/g film. Furthermore, the films with nettle extract boast an UV shielding ability with transmittance values close to zero in the UV region and a water solubility up to 1 %. The inherent biodegradability is also a strong advantage of the developed active films PB Elsevier SN 0141-8130 YR 2023 FD 2023 LK http://hdl.handle.net/10347/33242 UL http://hdl.handle.net/10347/33242 LA eng NO International Journal of Biological Macromolecules, Volume 253, Part 6, 2023, 127318 NO The authors appreciate the funding support of Xunta de Galicia, within the postdoctoral fellowship granted to Patricia Cazón Díaz (No. ED481B-2021-040). We acknowledge the use of RIAIDT-USC analytical facilities DS Minerva RD 24 abr 2026