RT Journal Article
T1 Resource allocation explains lactic acid production in mixed‐culture anaerobic fermentations
A1 Regueira López, Alberte
A1 Rombouts, Julius Laurens
A1 Wahl, S. Aljoscha
A1 Mauricio Iglesias, Miguel
A1 Lema Rodicio, Juan Manuel
A1 Kleerebezem, Robbert
K1 Auxotrophy
K1 Carboxylate platform
K1 Lactic acid bacteria
K1 Metabolic trade‐off
K1 Mixed microbial community
AB Lactate production in anaerobic carbohydrate fermentations with mixed cultures of microorganisms is generally observed only in very specific conditions: the reactor should be run discontinuously and peptides and B vitamins must be present in the culture medium as lactic acid bacteria (LAB) are typically auxotrophic for amino acids. State‐of‐the‐art anaerobic fermentation models assume that microorganisms optimise the adenosine triphosphate (ATP) yield on substrate and therefore they do not predict the less ATP efficient lactate production, which limits their application for designing lactate production in mixed‐culture fermentations. In this study, a metabolic model taking into account cellular resource allocation and limitation is proposed to predict and analyse under which conditions lactate production from glucose can be beneficial for microorganisms. The model uses a flux balances analysis approach incorporating additional constraints from the resource allocation theory and simulates glucose fermentation in a continuous reactor. This approach predicts lactate production is predicted at high dilution rates, provided that amino acids are in the culture medium. In minimal medium and lower dilution rates, mostly butyrate and no lactate is predicted. Auxotrophy for amino acids of LAB is identified to provide a competitive advantage in rich media because less resources need to be allocated for anabolic machinery and higher specific growth rates can be achieved. The Matlab™ codes required for performing the simulations presented in this study are available at https://doi.org/10.5281/zenodo.4031144
PB Wiley
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24315
UL http://hdl.handle.net/10347/24315
LA eng
NO Regueira, A, Rombouts, JL, Wahl, SA, Mauricio‐Iglesias, M, Lema, JM, Kleerebezem, R. Resource allocation explains lactic acid production in mixed‐culture anaerobic fermentations. Biotechnology and Bioengineering. 2021; 118: 745– 758. https://doi.org/10.1002/bit.27605
NO The authors would like to acknowledge the support of the Spanish Ministry of Education (FPU14/05457), project BIOCHEM (ERA‐IB‐2 7th call, ERA‐IB‐16‐052) funded by MINECO (PCIN 2016‐102) and the Soenghen Institute for Anaerobic Microbiology (SIAM), SIAM gravitation grant (the Netherlands Organization for Scientific Research, 024.002.002). Alberte Regueira would like to thank the CRETUS Strategic Partnership (ED431E 2018/01), for a research stay grant. Alberte Regueira, Miguel Mauricio‐Iglesias, and Juan M. Lema belong to the Galician Competitive Research Group (ED431C 2017/029) and to the CRETUS Strategic Partnership, both programmes are co‐funded by ERDF (EU)
DS Minerva
RD 28 abr 2026