RT Journal Article
T1 Combining antibacterial and wound healing features: Xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+
A1 Virzì, Nicola Filippo
A1 Díaz Rodríguez, Patricia
A1 Concheiro Nine, Ángel Joaquín
A1 Otero Casal, Ana María
A1 Mazzaglia, Antonino
A1 Pittalà, Valeria
A1 Álvarez Lorenzo, Carmen
K1 3D printing
K1 Anti-biofilm
K1 Thymol
K1 Zinc
K1 Isothermal microcalorimetry
K1 Xanthan gum
K1 Guar gum
K1 Cyclodextrin
AB Biofilm formation on biological and material surfaces represents a heavy health and economic burden for both patient and society. To contrast this phenomenon, medical devices combining antibacterial and pro-wound healing abilities are a promising strategy. In the present work, Xanthan gum/Guar gum (XG/GG)-based scaffolds were tuned with thymol and Zn2+ to obtain wound dressings that combine antibacterial and antibiofilm properties and favour the healing process. The tuning process preserved the 3D extrusion-based printability of the XG/GG ink. Scaffolds swelling profile was assessed in PBS pH 7.4, and the resistance to compressive forces was studied using a texturometer. The scaffolds microarchitectures were analyzed by SEM, while ATR-FTIR spotlighted the chemical modifications of the customized materials. Thymol and Zn2+ release was analyzed in biologically relevant media, showing a burst release in the first hours. The antibacterial properties were confirmed against S. aureus, P. aeruginosa, and S. epidermidis by isothermal microcalorimetry and biofilm viable cell counting. Incorporation of hydroxypropyl-β-cyclodextrin (HPβCD) improved thymol loading (7- and 14- times higher thymol content) and enhanced the antimicrobial and antioxidant performances of the dressing, while the presence of Zn2+ strongly potentiated the antimicrobial activity, showing a potent antibiofilm response in both Gram-positive and Gram-negative strains of clinical concern. The thymol and Zn2+ combination led to a reduction of 99.95 %, 99.99 %, and 98.26 %, of biofilm formation against S. aureus, P. aeruginosa, and S. epidermidis, respectively. Furthermore, the scaffolds demonstrated good hemocompatibility, cytocompatibility, tissue integration and pro-angiogenic features in an in ovo CAM model.
PB Elsevier
SN 0144-8617
YR 2025
FD 2025-03-01
LK https://hdl.handle.net/10347/42360
UL https://hdl.handle.net/10347/42360
LA eng
NO N.F. Virzì, P. Diaz-Rodriguez, A. Concheiro, A. Otero, A. Mazzaglia, V. Pittalà, C. Alvarez-Lorenzo. (2025). Combining antibacterial and wound healing features: xanthan gum/guar gum 3D-printed scaffold tuned with hydroxypropyl-β-cyclodextrin/thymol and Zn2+. "Carbohydrate Polymers", vol. 351, 1-19. https://doi.org/10.1016/j.carbpol.2024.123069
NO The work was supported by Spain Ministerio de Ciencia e Innovación MCIN/AEI/10.13039/501100011033 [PID2023-150422OB-I00], Xunta de Galicia [ED431C 2024/09], FEDER, and Italian PON Project BONE++ Development of Micro and Nanotechnologies for Predictivity, Diagnosis, Therapy and Regenerative Treatments of Pathological Bone and Osteo-Articular Alterations [ARS01_00693]. Work partially financed by project IBEROS+ [0072_IBEROS_MAIS_1_E] Interreg-POCTEP 2021–2027.
DS Minerva
RD 24 abr 2026