RT Journal Article
T1 Operation of a two-phase partitioning bioreactor for the oxidation of anthracene by the enzyme manganese peroxidase
A1 Eibes González, Gemma María
A1 Moreira Vilar, María Teresa
A1 Feijoo Costa, Gumersindo
A1 Daugulis, A. J.
A1 Lema Rodicio, Juan Manuel
K1 Biphasic reactor
K1 Anthracene
K1 Enzymatic oxidation
K1 Manganese peroxidase
AB A study was conducted to determine the potential of a two-phase partitioning bioreactor (TPPB) for the treatment of a poorly soluble compound, anthracene, by the enzyme manganese peroxidase (MnP) from the fungus Bjerkandera sp. BOS55. Silicone oil was used as the immiscible solvent, which contained anthracene at high concentrations. The optimization of the oxidation process was conducted taking into account the factors which may directly affect the MnP catalytic cycle (the concentration of H2O2 and malonic acid) and those that affect the mass transfer of anthracene between the organic and the aqueous phase (solvent and agitation speed). The main objective was carried out in terms of improved efficiency, i.e., maximizing the anthracene oxidized per unit of enzyme used. The TPPB reached nearly complete oxidation of anthracene at a conversion rate of 1.8 mg l−1 h−1 in 56 h, which suggests the application of enzymatic TPPBs for the removal of poorly soluble compounds.
PB Elsevier
SN 0045-6535
YR 2007
FD 2007-01
LK https://hdl.handle.net/10347/39250
UL https://hdl.handle.net/10347/39250
LA eng
NO G. Eibes, M.T. Moreira, G. Feijoo, A.J. Daugulis, J.M. Lema, Operation of a two-phase partitioning bioreactor for the oxidation of anthracene by the enzyme manganese peroxidase, Chemosphere, Volume 66, Issue 9, 2007, Pages 1744-1751, ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2006.07.009.
DS Minerva
RD 24 abr 2026