RT Journal Article
T1 In vitro assessment of emerging mycotoxins co-occurring in cheese: a potential health hazard
A1 Pérez Fuentes, Nadia
A1 Alvariño Romero, Rebeca
A1 Alfonso Rancaño, María Amparo
A1 González Jartín, Jesús María
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Roquefortine C
K1 Cyclopiazonic acid
K1 Toxicity
K1 Enniatins
K1 Apoptosis
K1 Synergism
AB Some Penicillium strains used in cheese ripening produce emerging mycotoxins, notably roquefortine C (ROQC) and cyclopiazonic acid (CPA), as well as enniatins (ENNs) and beauvericin (BEA). Co-occurrence of these mycotoxins in natural samples has been reported worldwide, however, most studies focus on the toxicity of a single mycotoxin. In the present study, the effects of ROQC and CPA alone and in combination with BEA and ENNs A, A1, B, and B1 were analysed in human neuroblastoma cells. ROQC and CPA reduced cell viability, with IC50 values of 49.5 and 7.3 µM, respectively, and induced caspase-8-mediated apoptosis. When ROQC and CPA were binary combined with ENNs, an enhancement of their individual effects was observed. Furthermore, a clear synergism was produced when ROQC and CPA were mixed with the four ENNs. An additive effect was also described for the combination of CPA + ENNs (A, A1, B, B1) + BEA. Finally, the effects of commercial cheese extracts containing the mentioned mycotoxins were evaluated, finding a strong reduction in cell viability. These results suggest that the co-occurrence of emerging mycotoxins in natural matrices could pose a potential health risk.
PB Springer
SN 0340-5761
YR 2024
FD 2024-09-25
LK https://hdl.handle.net/10347/42497
UL https://hdl.handle.net/10347/42497
LA eng
NO Pérez-Fuentes, N., Alvariño, R., Alfonso, A. et al. In vitro assessment of emerging mycotoxins co-occurring in cheese: a potential health hazard. Arch Toxicol 98, 4173–4186 (2024). https://doi.org/10.1007/s00204-024-03872-6
NO This work was supported by Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, GRC (ED431C 2021/01), Ministerio de Ciencia e Innovación, PID 2020-11262RB-C21, Grant CPP2021-008447,Luis M. Botana,European Commission, Interreg EAPA-0032/2022 – BEAP-MAR, Luis M. Botana, HORIZON-MSCA-2022-DN-01-MSCA Doctoral Networks 2022 101119901-BIOTOXDoc, Luis M. Botana, HORIZON-CL6-2023-CIRCBIO-01 COMBO—101135438, Luis M. Botana.
DS Minerva
RD 27 abr 2026