RT Journal Article
T1 In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells
A1 López García, Sergio
A1 Pecci Lloret, María Pilar
A1 Pecci Lloret, Miguel R.
A1 Oñate Sánchez, Ricardo E.
A1 García Bernal, David
A1 Castelo Baz, Pablo
A1 Rodríguez Lozano, Francisco Javier
A1 Guerrero Gironés, Julia
K1 Dental materials
K1 Cytotoxicity
K1 Biological properties
K1 Dental pulp cells
K1 Bioactive materials
AB This study aimed to analyze the biological effects of three new bioactive materials on cell survival, migration, morphology, and attachment in vitro. ACTIVA Kids BioACTIVE Restorative (Pulpdent, Watertown, MA, USA) (Activa), Ionolux (Voco, Cuxhaven, Germany), and Riva Light Cure UV (SDI, Bayswater, Australia) (Riva) were handled and conditioned with a serum-free culture medium. Stem cells from human dental pulp (hDPSCs) were exposed to material extracts, and metabolic activity, cell migration, and cell morphology were evaluated. Cell adhesion to the different materials was analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was evaluated by energy-dispersive X-ray (EDX). One-way analysis of variance followed by a Tukey test was performed (p < 0.05). Ionolux promoted a drastic reduction in metabolic activity and wound closure compared to the control (p < 0.05), whereas Activa induced adequate metabolic activity and cell migration. Moreover, SEM and immunofluorescence analysis showed abundant cells exposed to Activa. The materials showed different surface morphologies, and EDX spectra exhibited different peaks of C, O, Si, S, Ca, and F ions in glass ionomer cements. The results showed that Activa induced cell migration, cell attachment, and cell viability to a greater extent than Riva and Ionolux.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21676
UL http://hdl.handle.net/10347/21676
LA eng
NO López-García, S., Pecci-Lloret, M. P., Pecci-Lloret, M. R., Oñate-Sánchez, R. E., García-Bernal, D., Castelo-Baz, P., Rodríguez-Lozano, F. J., et al. (2019). In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells. Materials, 12(22), 3694. MDPI AG. Retrieved from http://dx.doi.org/10.3390/ma12223694
NO The Spanish Net of Cell Therapy (TerCel), RETICS subprograms of the I+D+I 2013-2016 Spanish National Plan, and project “RD16/0011/0001” funded by the Instituto de Salud Carlos III to JMM and co-funded by the European Regional Development Fund supported this work
DS Minerva
RD 3 may 2026