RT Journal Article
T1 Construction of a novel Pichia pastoris strain for production of xanthophylls
A1 Araya Garay, José Miguel
A1 Martínez Ageitos, José Manuel
A1 Vallejo Vidal, Juan Andrés
A1 Veiga Crespo, Patricia
A1 Sánchez Pérez, Ángeles
A1 González Villa, Tomás
K1 Pichia pastoris
K1 Carotenoids
K1 β-carotene
K1 Astaxanthin
AB In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-strains harboring astaxanthin-encoding genes from carotenogenic microorganism, and thus capable of producing xanthophyllic compounds. We designed and constructed a plasmid (pGAPZA-WZ) containing both the β-carotene ketolase (crtW) and β-carotene hydroxylase (crtZ) genes from Agrobacterium aurantiacum, under the control of the GAP promoter and containing an AOX-1 terminator. The plasmid was then integrated into the P. pastoris Pp-EBIL strain genomic DNA, producing clone Pp-EBILWZ. The recombinant P. pastoris (Pp-EBILWZ) cells exhibited a strong reddish carotenoid coloration and were confirmed, by HPLC, to produce not only the previous described carotenoids lycopene and β-carotene, but also de novo synthesized astaxanthin.
PB BioMed Central
YR 2012
FD 2012
LK http://hdl.handle.net/10347/21959
UL http://hdl.handle.net/10347/21959
LA eng
NO Araya-Garay, J.M., Ageitos, J.M., Vallejo, J.A. et al. Construction of a novel Pichia pastoris strain for production of xanthophylls. AMB Expr 2, 24 (2012). https://doi.org/10.1186/2191-0855-2-24
NO J. M. A-G. is the recipient of an AECID scholarship from the Spanish Foreign Affairs Ministry
DS Minerva
RD 24 abr 2026