RT Journal Article
T1 Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures
A1 Rombouts, Julius Laurens
A1 Kranendonk, Elsemiek Madeleine Maria
A1 Regueira López, Alberte
A1 Weissbrodt, David Gregory
A1 Kleerebezem, Robbert
A1 Loosdrecht, Mark Cornelis Maria van
K1 Enrichment cultures
K1 Kinetics
K1 Lactic acid bacteria
K1 Microbial ecology
K1 Resource allocation
AB Lactic acid‐producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology‐based process design and can aid the development of sustainable and energy‐efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose‐based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid‐producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes
PB Wiley
YR 2020
FD 2020
LK http://hdl.handle.net/10347/21669
UL http://hdl.handle.net/10347/21669
LA eng
NO Rombouts, JL, Kranendonk, EMM, Regueira, A, Weissbrodt, DG, Kleerebezem, R, van Loosdrecht, MCM. Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures. Biotechnology and Bioengineering. 2020; 117: 1281– 1293. https://doi.org/10.1002/bit.27301
NO Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Grant/Award Number: 024.002.002; Spanish Ministry of Education, Grant/Award Number: FPU14/05457
DS Minerva
RD 24 abr 2026