RT Journal Article
T1 Evidence of population structuring following population genetic analyses of Fasciola hepatica from Argentina
A1 Beesley, Nicola
A1 Attree, Elizabeth
A1 Vázquez Prieto, Severo
A1 Vilas Peteiro, Román
A1 Paniagua Crespo, Esperanza
A1 Martínez Ubeira, Florencio
A1 Jensen, Oscar
A1 Pruzzo, Cesar
A1 Álvarez, José D.
A1 Malandrini, Jorge Bruno
A1 Solana, Hugo Daniel
A1 Hodgkinson, Jane E.
K1 Fasciola hepatica
K1 Population genetics
K1 Population structure
K1 Clones
K1 Argentina
AB Fasciola hepatica, the liver fluke, is a trematode parasite that causes disease of economic importance in livestock. As a zoonosis this parasite also poses a risk to human health in areas where it is endemic. Population genetic studies can reveal the mechanisms responsible for genetic structuring (non-panmixia) within parasite populations and provide valuable insights into population dynamics, which in turn enables theoretical predictions of evolutionary dynamics such as the evolution of drug resistance. Here we genotyped 320 F. hepatica collected from 14 definitive hosts from four provinces in Argentina. STRUCTURE analysis indicated three population clusters, and principal coordinate analysis confirmed this, showing population clustering across provinces. Similarly, pairwise FST values amongst all four provinces were significant, with standardised pairwise FST (F′ST) ranging from 0.0754 to 0.6327. Therefore, population genetic structure was evident across these four provinces in Argentina. However, there was no evidence of deviation from Hardy–Weinberg equilibrium, so it appears that within these sub-populations there is largely random mating. We identified 263 unique genotypes, which gave a clonal diversity of 82%. Parasites with identical genotypes, clones, accounted for 26.6% of the parasites studied and were found in 12 of the 14 hosts studied, suggesting some clonemate transmission
PB Elsevier
SN 0020-7519
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26771
UL http://hdl.handle.net/10347/26771
LA eng
NO International Journal for Parasitology 2021, 51(6): 471-480. https://doi.org/10.1016/j.ijpara.2020.11.007
NO We are grateful for the time and expertise of an anonymous reviewer, who enhanced the accuracy and interpretation of this manuscript. This work was supported by Grant RTA2017-00010-C02-01 from the Ministerio de Economía, Industria y Competitividad (INIA, Spain), the Biotechnology and Biological Sciences Research Council, UK (BB/P001912/1) and the Institute of Infection and Global Health, University of Liverpool, UK. S.V.P. was supported by a postdoctoral fellowship from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. H. Solana is Principal Professional from the Comisión de Investigaciones Científicas (CIC-BA) de la Provincia de Buenos Aires, Argentina. Research at the Laboratorio de Biología Celular y Molecular is supported by SECAT-UNCPBA and CIVETAN CONICET, both of Universidad Nacional del Centro de la Provincia de Buenos Aires and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT PICT 2012/ N° 865-Préstamo BID) (all Argentina) and Contrato N°:017-2012-CONCYTEC-OAJ (CONCYTEC–Perú)
DS Minerva
RD 24 abr 2026