RT Journal Article
T1 Quantitative proteomics in medication-related osteonecrosis of the jaw: a proof-of-concept study
A1 Lorenzo Pouso, Alejandro Ismael
A1 Bravo Lopez, Susana Belén
A1 Carballo García, Francisco Javier
A1 Chantada Vázquez, María del Pilar
A1 Bagán Sebastián, José Vicente
A1 Bagán Debon, Leticia María
A1 Chamorro Petronacci, Cintia Micaela
A1 Conde Amboage, Mercedes
A1 López López, Rafael
A1 García García, Abel
A1 Pérez-Sayáns García, Mario
K1 Biomarker discovery
K1 MRONJ
K1 Prevention
K1 Prognosis
K1 Proteomics
K1 Saliva
AB Objective: Medication-related osteonecrosis of the jaw (MRONJ) is a paradoxical effect associated with bone-modifying agents (BMAs) and other drugs. Currently, no valuable diagnostic or prognosis biomarkers exist. The goal of this research was to study MRONJ-related salivary proteome. Materials and Methods: This case–control aimed to study salivary proteome in MRONJ versus control groups (i) formed from BMAs consumers and (ii) healthy individuals to unravel biomarkers. Thirty-eight samples of unstimulated whole saliva (18 MRONJ patients, 10 BMA consumers, and 10 healthy controls) were collected. Proteomic analysis by SWATH-MS coupled with bioinformatics analysis was executed. ResultsA total of 586 proteins were identified, 175 proteins showed significant differences among MRONJ versus controls. SWATH-MS revealed differentially expressed proteins among three groups, which have never been isolated. These proteins had distinct roles including cell envelope organization, positive regulation of vesicle fusion, positive regulation of receptor binding, or regulation of low-density lipoprotein particle clearance. Integrative analysis prioritized 3 proteins (MMP9, AACT, and HBD). Under receiver-operating characteristic analysis, this panel discriminated MRONJ with a sensitivity of 90% and a specificity of 78.9%. Conclusion: These findings may inform a novel biomarker panel for MRONJ prediction or diagnosis. Nonetheless, further research is needed to validate this panel
PB Wiley
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29271
UL http://hdl.handle.net/10347/29271
LA eng
NO Lorenzo-Pouso, A. I., Bravo, S. B., Carballo, J., del Chantada-Vázquez, M. P., Bagán, J., Bagán, L., Chamorro-Petronacci, C. M., Conde-Amboage, M., López-López, R., García-García, A., & Pérez-Sayáns, M. (2022). Quantitative proteomics in medication-related osteonecrosis of the jaw: a proof-of-  concept study. Oral Diseases, 00, 1–  13. https://doi.org/10.1111/odi.14201
DS Minerva
RD 24 abr 2026