RT Dissertation/Thesis
T1 New biomaterials for the design of nanomedicines that modulate glioblastoma stem cells
A1 García Mazás, Carla
K1 Gene therapy
K1 Polymeric Nanoparticles
K1 Polyphosphazene
K1 Glioblastoma
AB Gene therapy emerged in the 70s as an alternative to small drugs and proteins in the treatment of a large variety of diseases, but the administration of nucleic acids still remains a challenge due to the biological barriers that need to be overcome before reaching the target cells. Indeed, polynucleotides are very sensitive to degradation and cannot cross cell membranes. To overcome these obstacles, nucleic acids are often included in viral, lipid or polymeric particles. The objective of the thesis has been the development of new prototypes of polymeric nanoparticles for their use in gene therapy and to test their potential for the treatment of glioblastoma. For this, a variety of commercially available and synthetic cationic polymers have been combined with plasmid DNA and an endosomolytic polymer, nanoparticles were characterized for their physicochemical properties, for their toxicity and transfection efficiency in cell cultures. The prototype with better results regarding their transfection/toxicity ratio was used with a therapeutic plasmid encoding Bone Morphogenic Protein 4 as a potential treatment in vitro and in vivo against glioblastoma.
YR 2021
FD 2021
LK http://hdl.handle.net/10347/25173
UL http://hdl.handle.net/10347/25173
LA eng
DS Minerva
RD 23 abr 2026