RT Dissertation/Thesis
T1 Miniaturized devices for DNA analysis of food and environmental samples
A1 Carvalho, Joana Raquel Rodrigues
K1 microfluidics
K1 molecular biology
K1 food quality and safety
K1 food authenticity
K1 DNA analysis
K1 invasive species
AB Micro and nanotechnologies have been contributing to revolutionize manyindustry sectors. For DNA-based analysis, the major driving forces for developing new analytical strategies arereduced hands-on-time, increased sensitivity, multiplexing and faster methods. Consequently, there has been astrong interest in the miniaturization of such techniques. This Thesis combines these emerging technologies atmicroscale with well-established molecular biology techniques, using a modular approach to improve the steps ofDNA analysis: extraction, amplification and detection. Miniaturization allows automatization of the procedure withless reagent consumption, as well as lower energy consumption, facilitating on-site target DNA detection. The maingoal of this work was the development of miniaturized DNA analysis devices for food and environmental samples,providing efficient tools for a simpler, faster and less expensive analysis. Despite its advantages, miniaturized DNAanalysis devices have had limited diffusion into food and environmental sectors when compared to clinical andforensic applications. With this in mind, application in the food sector was particularly focused on food authenticityand allergen detection, and application in the environmental sector was focused on the detection of invasivespecies in river water. The development and optimization of such devices can contribute as new solutions to thefood industry and to environmental control.
YR 2022
FD 2022
LK http://hdl.handle.net/10347/28857
UL http://hdl.handle.net/10347/28857
LA eng
DS Minerva
RD 28 abr 2026