RT Journal Article
T1 NK-lysin peptides ameliorate viral encephalopathy and retinopathy disease signs and provide partial protection against nodavirus infection in European sea bass
A1 Valero Cuesta, Yulema
A1 González Fernández, Carmen
A1 Cárdenas, Constanza
A1 Guzmán, Fanny
A1 León, Rosa
A1 Cuesta, Alberto
K1 Antimicrobial peptides (AMP)
K1 NK-lysin
K1 Nervous necrosis virus
K1 European sea bass
K1 Teleost fish
AB Antimicrobial peptides (AMP) comprise a wide range of small molecules with direct antibacterial activity and immunostimulatory role and are proposed as promising substitutes of the antibiotics. Additionally, they also exert a role against other pathogens such as viruses and fungi less evaluated. NK-lysin, a human granulysin orthologue, possess a double function, taking part in the innate immunity as AMP and also as direct effector in the cell-mediated cytotoxic (CMC) response. This molecule is suggested as a pivotal molecule involved in the defence upon nervous necrosis virus (NNV), an epizootic virus provoking serious problems in welfare and health status in Asian and Mediterranean fish destined to human consumption. Having proved that NK-lysin derived peptides (NKLPs) have a direct antiviral activity against NNV in vitro, we aimed to evaluate their potential use as a prophylactic treatment for European sea bass (Dicentrarchus labrax), one of the most susceptible cultured-fish species. Thus, intramuscular injection of synthetic NKLPs resulted in a very low transcriptional response of some innate and adaptive immune markers. However, the injection of NKLPs ameliorated disease signs and increased fish survival upon challenge with pathogenic NNV. Although NKLPs showed promising results in treatments against NNV, more efforts are needed to understand their mechanisms of action and their applicability to the aquaculture industry
PB Elsevier
SN 0166-3542
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26512
UL http://hdl.handle.net/10347/26512
LA eng
NO Antiviral Research, 192 (2021), 105104. https://doi.org/10.1016/j.antiviral.2021.105104
NO This work was supported by Ministerio de Economía y Competitividad (MINECO; grant AGL2016-74866-C3-1-R with FEDER co-funds), Agencia Española de Investigación (PID2019-105522 GB-I00), Fundación Séneca, Grupo de Excelencia de la Región de Murcia (grant 19883/GERM/15) and National Commission for Scientific & Technological Research Chile (grant FONDECYT N° 1170379)
DS Minerva
RD 1 may 2026