RT Journal Article
T1 Transcriptomic Analysis of Ciguatoxin-Induced Changes in Gene Expression in Primary Cultures of Mice Cortical Neurons
A1 Rubiolo Gaytán, Juan Andrés
A1 Vale González, María del Carmen
A1 Boente Juncal, Andrea
A1 Hirama, Masahiro
A1 Yamashita, Shuji
A1 Camiña García, María Mercedes
A1 Rodríguez Vieytes, Mercedes
A1 Botana López, Luis Miguel
K1 Ciguatoxin
K1 Ciguatera
K1 Cortical neuron
K1 Gene
K1 Tetrodotoxin
AB Ciguatoxins are polyether marine toxins that act as sodium channel activators. These toxins cause ciguatera, one of the most widespread nonbacterial forms of food poisoning, which presents several symptoms in humans including long-term neurological alterations. Earlier work has shown that both acute and chronic exposure of primary cortical neurons to synthetic ciguatoxin CTX3C have profound impacts on neuronal function. Thus, the present work aimed to identify relevant neuronal genes and metabolic pathways that could be altered by ciguatoxin exposure. To study the effect of ciguatoxins in primary neurons in culture, we performed a transcriptomic analysis using whole mouse genome microarrays, for primary cortical neurons exposed during 6, 24, or 72 h in culture to CTX3C. Here, we have shown that the effects of the toxin on gene expression differ with the exposure time. The results presented here have identified several relevant genes and pathways related to the effect of ciguatoxins on neurons and may assist in future research or even treatment of ciguatera. Moreover, we demonstrated that the effects of the toxin on gene expression were exclusively consequential of its action as a voltage-gated sodium channel activator, since all the effects of CTX3C were avoided by preincubation of the neurons with the sodium channel blocker tetrodotoxin
PB MDPI
YR 2018
FD 2018
LK http://hdl.handle.net/10347/23596
UL http://hdl.handle.net/10347/23596
LA eng
NO Rubiolo, J.A.; Vale, C.; Boente-Juncal, A.; Hirama, M.; Yamashita, S.; Camiña, M.; Vieytes, M.R.; Botana, L.M. Transcriptomic Analysis of Ciguatoxin-Induced Changes in Gene Expression in Primary Cultures of Mice Cortical Neurons. Toxins 2018, 10, 192
NO The research leading to these results has received funding from the following FEDER cofounded-grants. From CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad, AGL2014-58210-R, AGL2016-78728-R (AEI/FEDER, UE), ISCIII/PI16/01830 and RTC-2016-5507-2. From CDTI under ISIP Programme, Spain, IDI-20130304 APTAFOOD and ITC-20161072. From European Union POCTEP 0161-Nanoeaters -1-E-1, and Interreg AlertoxNet EAPA-317-2016. ABJ is recipient of a predoctoral fellowship from the Spanish Ministry of Education
DS Minerva
RD 30 abr 2026