RT Journal Article
T1 Complementarity of two approaches based on the use of high-resolution mass spectrometry for the determination of multi-class antibiotics in water. Photodegradation studies and non-target screenings
A1 Vázquez Ferreiro, Lúa
A1 Llompart Vizoso, María del Pilar
A1 Dagnac, Thierry
K1 Antibiotics
K1 Solid-phase extraction
K1 UHPLC-QToF-HRMS
K1 Non-target analysis
K1 Photodegradation
K1 Photoproducts
AB The development of analytical methodologies to monitor different antibiotic families in water and the implementation of alternatives for their efficient elimination are a great challenge. The aim of this research was to develop a method based on solid-phase extraction followed by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry to analyse multi-class antibiotics, including macrolides, cephalosporins, fluoroquinolones, sulfonamides and diaminopyrimidines, in waters. Several parameters affecting the extraction such as the sample pH, type of sorbent and cartridge, elution volume and breakthrough volume were optimized. The method was validated in real samples, and matrix effect was assessed, demonstrating that the use of isotopically labelled surrogate compounds was mandatory to avoid standard addition calibration for each individual samples. Urban and hospital wastewater samples, as well as natural waters, were analysed, confirming the presence of 12 of the 14 target compounds at concentrations up to 3.5 µg L−1. Non-target analysis based on data-independent workflow was also performed, enabling the identification of 94 pollutants. Preliminary photodegradation experiments were also assessed, revealing the total removal of many target compounds after the first 5–10 min of UVC irradiation. In addition, 20 by-products formed after photolysis could be identified using a non-target approach
PB Springer
SN 0944-1344
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29180
UL http://hdl.handle.net/10347/29180
LA eng
NO Environmental Science and Pollution Research (2022). https://doi.org/10.1007/s11356-022-22130-9
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was supported by projects EQC2018-005011-P (Ministry of Science, Innovation and Universities, Spain) and IGDC-1E-2026 (Infrastructure Program, MINECO, Spain). The authors belong to the National Network for the Innovation in miniaturized sample preparation techniques, RED2018-102522-T (Ministry of Science, Innovation and Universities, Spain) and to the Galician Competitive Research Groups IN607B 2019/13 and ED431 2020/06 (Xunta de Galicia). This study is based upon work from the Sample Preparation Study Group and Network, supported by the Division of Analytical Chemistry of the European Chemical Society. All these programmes are co-funded by FEDER (EU)
DS Minerva
RD 24 abr 2026