RT Journal Article
T1 Neuroprotective effects of fluorophore-labelled manganese complexes: Determination of ROS production, mitochondrial membrane potential and confocal fluorescence microscopy studies in neuroblastoma cells
A1 Rouco, Lara
A1 Alvariño Romero, Rebeca
A1 Alfonso Rancaño, María Amparo
A1 Pedrido Castiñeiras, Rosa
A1 Maneiro Maneiro, Marcelino
A1 Romero Castro, María José
K1 Manganese complexes
K1 Antioxidant properties
K1 Human neuroblastoma cells
AB In this work, four manganese(II) complexes derived from the ligands H2L1-H2L4, that incorporate dansyl or tosyl fluorescent dyes, have been investigated in term of their antioxidant properties. Two of the manganese(II) complexes have been newly prepared using the asymmetric half-salen ligand H2L2 and the thiosemicarbazone ligand H2L3. The four organic strands and the manganese complexes have been characterized by different analytical and spectroscopic techniques. The study of the antioxidant behaviour of these two new complexes and other two fluorophore-labelled analogues was tested in SH-SY5Y neuroblastoma cells. These four model complexes 1–4 were found to protect cells from oxidative damage in this human neuronal model, by reducing the release of reactive oxygen species. Complexes 1–4 significantly improved cell survival, with levels between 79.1 ±0.8% and 130.9 ±4.1%. Moreover, complexes 3 and 4 were able to restore the mitochondrial membrane potential at 1 μM, with 4 reaching levels higher than 85%, similar to the percentages obtained by the positive control agent cyclosporin A. The incorporation of the fluorescent label in the complexes allowed the study of their ability to enter the human neuroblastoma cells by confocal microscopy.
PB Elsevier
SN 0162-0134
YR 2022
FD 2022
LK http://hdl.handle.net/10347/32354
UL http://hdl.handle.net/10347/32354
LA eng
NO Rouco, L., Alvariño, R., Alfonso, A., Romero, M. J., Pedrido, R., & Maneiro, M. (2022). Neuroprotective effects of fluorophore-labelled manganese complexes: Determination of ROS production, mitochondrial membrane potential and confocal fluorescence microscopy studies in neuroblastoma cells. Journal of Inorganic Biochemistry, 227, 111670.
NO The research leading to these results has received funding from the following FEDER cofunded-grants. From Consellería de Cultura, Educaci´ on e Ordenaci´ on Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01), 2018 GRC GI-1584 (ED431C 2018/13), MetalBIO Network (ED431D 2017/01). From CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad IISCIII/PI19/001248. From Ministerio de Ciencia, Innovaci´ on y Universidades, MULTIMETDRUGS (RED2018-102471-T). From European Union, Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA- 998-2018, and H2020 778069-EMERTOX.
DS Minerva
RD 23 abr 2026