RT Journal Article
T1 Assessing the Effect of Surface Coating on the Stability, Degradation, Toxicity and Cell Endocytosis/Exocytosis of Upconverting Nanoparticles
A1 Arellano, Lilia
A1 Martínez, Raquel
A1 Pardo Montero, Alberto
A1 Díez, Iago
A1 Velasco Rodríguez, Brenda
A1 Moreda Piñeiro, Antonio
A1 Bermejo Barrera, Pilar
A1 Barbosa Fernández, Silvia
A1 Taboada Antelo, Pablo
K1 Up-converting nanoparticles
K1 Surface coating
K1 Stability and degradation
K1 Endocytosis/exocytosis
AB Lanthanide-doped up-converting nanoparticles (UCNPs) have emerged as promising biomedical tools in recent years. Most research efforts were devoted to the synthesis of inorganic cores with the optimal physicochemical properties. However, the careful design of UCNPs with the adequate surface coating to optimize their biological performance still remains a significant challenge. Here, we propose the functionalization of UCNPs with four distinct types of surface coatings, which were compared in terms of the provided colloidal stability and resistance to degradation in different biological-relevant media, including commonly avoided analysis in acidic lysosomal-mimicking fluids. Moreover, the influence of the type of particle surface coating on cell cytotoxicity and endocytosis/exocytosis was also evaluated. The obtained results demonstrated that the functionalization of UCNPs with poly(isobutylene-alt-maleic anhydride) grafted with dodecylamine (PMA-g-dodecyl) constitutes an outstanding strategy for their subsequent biomedical application, whereas poly(ethylene glycol) (PEG) coating, although suitable for colloidal stability purposes, hinders extensive cell internalization. Conversely, surface coating with small ligand were found not to be suitable, leading to large degradation degrees of UCNPs. The analysis of particle’ behavior in different biological media and in vitro conditions here performed pretends to help researchers to improve the design and implementation of UCNPs as theranostic nanotools.
PB Elsevier
SN 0021-9797
YR 2024
FD 2024
LK http://hdl.handle.net/10347/34907
UL http://hdl.handle.net/10347/34907
LA eng
NO Journal of Colloid and Interface Science Volume 668 , 15 August 2024, Pages 575-586
NO This research was funded by Agencia Estatal de Investigación (project number PID2019-109517RB-I00), and Xunta de Galicia (ED431C 2022/18 and ED481B2019/025). ERDF funds are also acknowledged.
DS Minerva
RD 24 abr 2026